Quality Testing/Characterization
Synthesized RNA oligonucleotides must be characterized to ensure synthesis reactions are proceeding as expected. Check reaction yields and efficiency by measuring absorbance on aMolecular Devices SpectraMax plate reader. Automate the preparation of sequencing libraries of oligonucleotides with a Beckman Coulter Life Sciences Biomek NGeniuS Next Generation Library Prep System. Array qPCR reactions and sequencing libraries quickly with a Beckman Coulter Life Sciences Echo Acoustic Liquid Handler.
Check oligo size and purity on aSCIEX PA800 Plus Pharmaceutical Analysis System or with a SCIEX Biophase 8800 Multi-capillary Electrophoresis System. Analyze with LC-MS by coupling a SCIEX Exion 2.0 HPLC systemwith a SCIEX ZenoTOF 7600 mass spectrometer to ensure synthesis reactions produce the correct nucleotide sequences down to the atomic level. For mass spectrometry applications with truly high numbers of molecules to characterize, a SCIEX Echo-MS System can be used to quickly array small quantities of molecules directly for mass confirmation. Capture and manage data with the IDBS Polar Biopharma Lifecycle Management platform. Streamline mass spectrometry data analysis with Genedata Expressionist Software.