Over time, they have gained a strong reputation for their dependability and user-friendliness. These systems can be customized according to factors like library size, detection mode, screening format, screening assay, and target, facilitating a smooth transition from initial assay development to the optimization of lead compounds. Our systems include imaging features that enable real-time visualization of cellular responses and function as plate readers, facilitating the measurement of cellular responses in multi-well plates for high-throughput screening applications.

Overview

Equipped with a high-speed EMCCD camera and an intutive software module, our latest high-throughput screening systemempowers you to capture and analyze peaks in human-induced pluripotent stem cells (hiPSCs) that have been differentiated into cardiomyocytes and neurons. This advanced system can record events at a remarkable rate of up to 100 times per second, enabling rapid identification of occurrences such as early afterdepolarization-like events (EAD-like events), fibrillation, and irregular beating. With their array of seven LED sets, numerous filter options, and the ability to detect fluorescence or luminescence, this system provides extensive support for a wide range of biochemical assays, including those related to calcium flux, potassium, and membrane potential. Our system uses software to set up and run experiments with ease, facilitating a drag-and-drop interface. It offers options like standard fluorescent or luminescent reading, complex liquid handling, kinetic cell-based readings, tip washing with two solvents, and simple automation protocols. This system is designed to seamlessly integrate with automation systems and are compatible with a wide range of reagents used in cellular assays, allowing for flexibility in experimental design and accommodating various research needs.

Features

Essential Features of our High Throughput Screening System

• The software provides a single platform for comprehensive data analysis and management of calcium oscillation peak data, allowing for the identification of compounds that might trigger waveform irregularities associated with arrhythmia, seizures, or other toxic effects, as well as the characterization of diverse calcium oscillations in neurons without the need for data export.
• You can easily switch pipette heads within minutes, without the need for tools, to accommodate different cell-based assay throughput requirements, whether it's for 96, 384, or 1536 channels.
• All the system components, including hardware, software, and high-throughput screening assay kits, have undergone validation by a highly respected industry leader with more than four decades of experience.
• These systems can be tailored to user requirements, beginning with a standard camera for cost-effective GPCR and membrane potential assays or a new option. These cameras can capture up to 100 images per second and deliver outstanding luminescence performance. The system offers flexibility to configure the camera to high speed, LEDs, and filters either at the time of purchase or later to adapt to changing assay needs.
• While drug discovery follows common methods, each project has its unique aspects, and our solution team provides a range of tailored services, including automated compound plate delivery and fully integrated workstations with automated incubation, to meet diverse project needs.

Applications

Applications of our High Throughput Screening Systems

• The cellular screening system can be applied in toxicology research, assessing the impact of chemicals on living organisms in our increasingly chemical-exposed world.
• It is used to evaluate the activity of potassium ion channels, especially in the context of cardiac safety in drug discovery, by measuring channel activity through thallium ion permeability and a sensitive indicator dye.
• The high-throughput cell screening technology plays a crucial role in addressing the high failure rate in drug development by enabling the assessment of drug candidates in more biologically relevant 3D cell cultures, helping to provide more accurate predictions of a drug's potential and reducing development timelines.
• The use of a high-throughput cellular screening system is essential for early prediction of drug-induced functional cardiotoxicity, as it facilitates high-throughput screening using readily available iPSC-derived human cardiomyocytes, calcium-sensitive dyes, and the analysis of calcium peaks with our software to quantitatively assess cardiomyocyte activity.
• While primarily designed for cellular screening, the system can be adapted for specific molecular assays, making it a versatile tool for various research applications.
• The system is well-suited for high-throughput screening of small molecules to evaluate their effects on cellular activities.

FAQs