Capillary Electrophoresis Instruments
P/ACE MDQ Plus system
CESI 8000 Plus ESI-MS System
PA 800 Plus Pharmaceutical Analysis System
BioPhase™ 8800 system
Product Highlights/Key Features
Our capillary electrophoresis instruments that allow for capillary gel electrophoresis are equipped with advanced features to level up your protein, viral vector, messenger RNA (mRNA) or DNA analysis workflows.
- Versatility: To enable the robustness for today and the flexibility for tomorrow, our CE systems are extremely versatile. Choose from protein purity analysis with capillary electrophoresis sodium dodecyl sulfate (CE-SDS), mRNA and viral vector genome integrity and purity, protein charge heterogeneity assessment with capillary isoelectric focusing (cIEF), released glycan characterization and more. Meeting high sensitivity needs for low sample amounts is not a problem with our systems’ ability to choose from multiple on-board detector modes including UV or high-sensitivity laser-induced fluorescence (LIF) detectors.
- Simplicity: Kit-based solutions combined with instrument and software let you reclaim your time and stay focused on the science through an end-to-end workflow.
- Reliability: To obtain reliable, accurate, and reproducible data, our capillaries are liquid cooled. Compared to air cooled systems, liquid cooling allows for more precise temperature control, keeping your %CVs tight.
- Efficiency: Time is of the essence. As is the quality of results. To help with the efficiency of your lab, our systems offer ultra high-resolution analysis of up to 8 samples in parallel and instruments that can work in higher-throughput settings or lower-throughput settings, where critical analysis is a must.
- QC-readiness: Industry-trusted compliance features and compatibility with chromatography data systems help you meet global regulatory requirements.
Application of capillary electrophoresis equipment
The versatile nature of our capillary electrophoresis equipment and vast range of kits let you forge new paths and seamless workflows for your specific application needs.
- mRNA: Ensuring the quality and stability of the fragile in vitro transcribed RNA (IVT RNA) cargo is of utmost importance. Intuitive kits covering wide nucleic acid size ranges coupled to extremely high resolving power enable you to understand your mRNAs better and perform purity and integrity assessments with ease.
- Proteins: From monoclonal antibodies (mAb) and mAb variants to complex viral vectors, the high-quality data obtained with our capillary electrophoresis systems help you characterize your molecules reliably each day.
- Plasmid DNA: The quality of plasmid DNA (pDNA) directly impacts the quality of subsequent protein, nucleic acid, or viral vector products. With intuitive kit-based solutions, you can assess pDNA topology and purity, perform linearization efficiency and sizing analyses and identify repetitive DNA sequences of your critical starting material with excellent separation power.
- Gene editing: CRISPR-based gene editing relies on guide RNAs and Cas9-encoding mRNAs. With liquid-temperature control, you can leave secondary structures impeding purity assessment of complex next-generation guide RNAs (i.e. prime editing guide RNAs, pegRNAs) in the past and focus on the future.
- Applied markets: Ensuring your products are meeting quality criteria does not have to be complicated. Whether you are looking at the whey proteins in your food product, the glycan distribution in baby formula glycans or anion/cation coatings of chips, our flexible CE systems help you prepare to answer whatever scientific question comes next.
FAQs
Different types of capillary electrophoresis include capillary gel electrophoresis (CGE), capillary isoelectric focusing (cIEF), capillary zone electrophoresis (CZE), microemulsion electrokinetic chromatography (MEEKC)and, capillary isotachophoresis (CITP).
The various types of detectors used in capillary electrophoresis are UV/vis absorption detectors, laser-induced fluorescence detectors, fluorescence detectors, mass spectrometers, radiometry detectors, conductivity detectors, and electrochemical detectors.
Capillary electrophoresis instruments offer several advantages:
- High-resolution separation of molecules and analytes
- Excellent reproducibility
- Automated analysis of samples with minimal hands-on time
- Reliable, software-based peak integration
- Variety of separation modes for different needs
When a high-voltage power supply or an electric field is applied to a capillary tube carrying the sample in buffer solution, the charged ions start moving to different ends of the capillary based on their charge and size. If two molecules are the same size, they separate based on the charge they carry. The cations move toward the negatively charged cathode, and the anions move toward the anode that is positively charged. In cases where two ions have the same charge, they move based on their size (for example, the smaller ion moves faster than the larger ion).