Our gene-edited cell lines, developed using CRISPR-Cas9 technology, precisely target and disrupt specific genes within the genome and are verified through Sanger sequencing. Knock-out (KO) cell lines provide reliable controls for assay development and facilitate precise analysis of genotype-phenotype relationships. However, generating CRISPR KO cell lines can be a lengthy process, often requiring three to four rounds of the CRISPR workflow to achieve the desired edits. To save you valuable time, we offer an extensive and growing selection of ready-to-use and custom-made KO cell lines in widely used models - making CRISPR KO cell lines as accessible as standard reagents. Rigorously validated for knock-out, they accelerate progress in target validation, pathway exploration, disease research, and beyond.
Confidently investigate the functional effects of your target with our expanding collection of gene-edited cell lines in physiologically relevant models, including A549 and MCF-7 cells. Our CRISPR KO cell line catalog streamlines your workflow, reducing preparation time by over four months. Instead of spending months on experimental setup and confirmation, you can obtain the KO cell line you need with a simple click - accelerating your research and helping you publish sooner.
Where available, we also offer wild-type cell lines as controls, ensuring reliable comparisons. With outstanding batch-to-batch consistency, our clonal gene-edited cell lines provide reproducible, high-quality results so you can focus on advancing your research.
Our cell lysates are obtained from cell lines using lysis buffers and can be used as positive or negative controls in western blot and ELISA, among other assays. Offering a more convenient alternative to live cell lines, they eliminate the need for cell culture and serve as an ideal tool when working with denatured proteins. Our cell lysates and cell lines are also valuable in research for the discovery and validation of biomarkers associated with certain diseases.
KO cell lysates are supplied alongside the corresponding parental wild-type lysate, ensuring a consistent cellular background for accurate assessment of the knock-out’s biological impact.
Get access to our extensive range of KO cell lines and cell lysates generated from a range of commonly used cell lines, including cancer cell lines.
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Features
- Instant availability: Eliminate the need to generate your own KO cell line, saving up to 12 weeks of research time.
- Extensive selection: Choose from the industry’s largest collection of immortalized diploid KO cell lysates, including HeLa, HEK293T, A549, HCT116, Hep G2, and MCF7, all derived from single-cell clones.
- Consistent quality: Each lysate is prepared using a standardized process, ensuring reproducibility and reliable experimental results.
- Extensive, easy-to-access catalog: Quickly find your target with our user-friendly catalog, featuring various key targets in multiple backgrounds, including physiologically relevant models like A549 and MCF-7.
- High-quality, validated cell lines: Our cell lines are sourced from certified mycoplasma-free backgrounds and undergo rigorous genomic validation using Sanger sequencing, with proteomic validation available for many to confirm full gene knock-out.
- Robust experimental controls: Every order includes control cell lines to ensure reliable, reproducible results, helping you maintain experimental consistency.
Applications
- These KO cell lines serve as essential tools for exploring disease mechanisms and understanding genotype-phenotype relationships, making them invaluable for discovering new therapeutic targets.
- Our cell lysates provide a reliable and high-quality source of proteins and biomolecules to facilitate disease-related biomarker identification and validation. Comparing lysates derived from our healthy and KO cells can allow you to detect differentially expressed proteins that may serve as potential diagnostic markers or therapeutic targets, supporting advancements in biomedical research and drug discovery.
- The advancements in CRISPR technology have driven the widespread use of gene-edited cells across various assays, accelerating drug discovery and optimizing assay development.
- Our KO cell lines can be used as a control to confirm antibody specificity and reduce background in imaging assays such as immunocytochemistry/immunofluorescence.
- They ensure accurate antibody binding and validate protein expression in flow cytometry.
- In western blot, they verify antibody specificity by confirming the absence of the target proteins.
- In immunocytochemistry, our KO cell lines help detect and confirm protein presence inside cells.
- In sandwich ELISA, they ensure that secreted proteins are completely knocked out for accurate detection.
- For immunohistochemistry, they validate antibody specificity in tissue staining experiments.
FAQs
What are lysates from the cell line?
Cell lysates are extracts obtained by breaking open cells using lysis buffers, preserving intracellular proteins, nucleic acids, and other biomolecules. They provide a convenient source of cellular components for various biochemical and analytical applications without requiring live cell culture.
What are the key considerations when selecting a cell lysate for western blotting?
When selecting a cell lysate for western blotting, it‘s important to choose one that expresses the target protein at detectable levels. Other factors include using matched KO and wild-type lysates as controls, ensuring the lysate is properly prepared to preserve protein integrity, and selecting appropriate lysis buffers to maintain protein solubility.
How can cell lysates be used for biomarker identification?
Cell lysates provide a rich source of proteins and other biomolecules, making them useful for identifying and validating disease-related biomarkers. By comparing lysates from healthy and diseased cells, researchers can detect differentially expressed proteins that may serve as diagnostic or therapeutic targets.
What types of assays can be performed using cell lysates?
Cell lysates are commonly used in western blotting, ELISA, immunoprecipitation, and mass spectrometry for protein analysis. They are also valuable in enzyme activity assays, protein-protein interaction studies, and next-generation sequencing applications to investigate cellular mechanisms.
What are the advantages of using whole cell lysates over fractionated lysates?
Whole-cell lysates provide a complete representation of all cellular proteins, allowing for a broad analysis of protein expression and interactions. They simplify sample preparation and are particularly useful when studying overall protein abundance, signaling pathways, or cellular responses without needing to isolate specific cellular compartments.