You can access a comprehensive range of separation technologies, including analytical flow liquid chromatography-mass spectrometry, microflow liquid chromatography-mass spectrometry, nanoflow liquid chromatography-mass spectrometry, as well as ultra-low flow capillary electrospray ionization mass spectrometry. Our systems ensure that your workflow demands are consistently met in terms of reproducibility, reliability, and the prevention of carryover between injections, regardless of the number of injections or batches.
Overview
Our HPLC systems ensure correct chromatography data flow. The integrated software ensures data integrity, smooth usability, and enhanced efficiency for your mass spectrometry workflows across your entire laboratory. These systems, built on our tradition of innovation, offer precision and durability while maintaining the quality and support you'd expect from the leader in molecule quantification. They seamlessly integrate, are fully adjustable, and work harmoniously with our cutting-edge mass spectrometers. These systems provide the speed and accuracy you anticipate, offering a complete solution for addressing any challenges your laboratory may encounter.
Our UHPLC systems, fully configured for high-demand liquid chromatography separations, are designed to effortlessly deliver flow rates of up to 5 mL/min at 18,000 PSI. These feature a high-pressure binary pump capable of reaching 12,500 PSI, an integrated four-channel degasser, and a standard solvent selection valve, making them a cost-effective solution for a wide range of applications. Specifically optimized for our microLC systems, our columns operate at high linear flow velocities to ensure optimal performance at increased speeds and throughput levels. Whether used as a standalone capillary electrophoresis instrument or in conjunction with the power of mass spectrometry, our systems allow you to focus on your scientific research rather than getting bogged down by technology. These enable the discovery of previously imperceptible characteristics from even the smallest and most valuable samples, all in record time.
Features
Essential features of our HPLC systems
- Our environmentally conscious systems strike a harmonious balance between sensitivity and durability, offering optional features like a wash system module, various detector choices, and switching valves.
- The user-friendly autosamplers are easy to operate and maintain, with scalable injection volumes ranging from 0.5 to 1000 microliters, multiple injection possibilities, and outstanding injection linearity and reproducibility.
- The UHPLC pumps come equipped with automatic purging, an integrated four-channel degasser, and a solvent selection valve. Engineered for improved stability, their innovative design ensures consistent, precise results day in and day out.
- Our HPLC columns offer exceptional chromatographic performance with a unique fingertight end-fitting system for easy installation, ensuring long-lasting column lifetime, and are available in various stationary phases and lengths.
- Incorporating capillary electrophoresis and electrospray ionization into a single dynamic process within the same device offers researchers minute sample consumption, streamlined workflows for multi-level characterization of protein therapeutics, and a frustration-free experience with open tube technology.
- A comprehensive selection of optical detectors seamlessly integrated with the MS solutions offers a wide spectrum of choices ranging from multi-wave to diode-array detection.
Applications
Applications of HPLC Systems
- In the pharmaceutical industry, HPLC systems are used to determine the concentration of therapeutic peptides in complex biological matrices like serum or plasma. This quantification is crucial in assessing the pharmacokinetics and bioavailability of therapeutic peptides, ensuring proper dosing and effectiveness of peptide-based drugs.
- HPLC systems equipped with microflow sensitivity are utilized to enhance small molecule and metabolite analysis, enabling the accurate quantification of trace levels of these compounds in various samples, which is particularly valuable in pharmaceutical research, clinical diagnostics, and environmental monitoring.
- HPLC systems are employed in cell line development to monitor and analyze the production and secretion of biologically active compounds.
- In antisense oligonucleotide development and manufacturing, HPLC systems are essential for characterizing and quantifying oligonucleotide purity, sequence integrity, and stability, thereby contributing to the production of reliable therapeutics.
- The systems are employed in pDNA quality control, verifying the integrity, purity, and concentration of the pDNA samples used in various biotechnological applications, such as gene therapy and vaccine development.
- The systems play a pivotal role in monoclonal antibody (mAb) development and production by quantifying mAb purity, characterizing glycosylation patterns, and assessing the stability of mAb formulations.
- HPLC systems are instrumental in mRNA development and manufacturing, where they are used to assess the quality, purity, and integrity of synthesized mRNA molecules, ensuring their suitability for applications such as mRNA-based therapies, vaccines, and gene expression studies.
FAQs
What is HPLC, and how does it work?
High-performance liquid chromatography (HPLC) is an analytical chemistry technique that separates and identifies components of a mixture by passing it through a column filled with a stationary phase and a mobile phase. It can operate in two primary modes:
- The normal phase, where polar components are retained by a polar stationary phase.
- The reversed phase, where non-polar components are separated using a non-polar stationary phase.
What is preparative HPLC, and how does it differ from analytical HPLC?
Preparative HPLC is a technique used to isolate and purify compounds in larger quantities compared to analytical HPLC. While analytical HPLC is primarily focused on identifying and quantifying compounds in a sample, preparative HPLC is designed for the separation and collection of target compounds for further use.
What is CESI-MS, and how does it help in discovering previously undetectable characteristics from small sample quantities?
CESI-MS stands for capillary electrophoresis with electrospray ionization mass spectrometry. It's an innovative analytical technique that combines the separation power of capillary electrophoresis with mass spectrometry detection. CESI-MS is highly sensitive and can reveal hidden information from even the smallest sample amounts by providing detailed molecular analysis.
What are the key advantages of the M5 MicroLC system's intelligent microflow technology?
The M5 MicroLC system offers greater sensitivity, flexibility, and robustness. It achieves increased ionization efficiency, provides dual flow path options to optimize analysis time, and features click-and-go fittings for reliable connections, ensuring ease of use and accurate results.
HPLC vs. GC: What's the difference?
High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are both chromatographic techniques used for separating and analyzing compounds, but they differ in the mobile phase they use. HPLC uses a liquid mobile phase (typically a solvent), while GC uses a gaseous mobile phase. This distinction leads to differences in the types of compounds each method is best suited for, with HPLC being more appropriate for polar and non-volatile compounds and GC being ideal for volatile and less polar compounds.
What are the latest advancements in HPLC technology?
Recent advancements in HPLC technology include the widespread adoption of Ultra High-Performance Liquid Chromatography (UHPLC) for faster separations, innovations in column technology for improved resolution, and the integration of advanced detectors such as mass spectrometry. Additionally, developments in miniaturization, automation, and hyphenated techniques contribute to enhanced sensitivity, efficiency, and versatility in analytical applications.
What are the ethical considerations for using HPLC systems?
The ethical considerations of using HPLC systems encompass maintaining research integrity, transparency in reporting, and responsible data sharing, especially in studies involving human or animal subjects. Researchers must also address environmental impact, disclose conflicts of interest, and promote accessibility to ensure ethical conduct and public trust in scientific research.
What are the key advantages of using HPLC systems in laboratories?
The key advantages of using HPLC systems in laboratories lie in the comprehensive range of separation technologies offered, including analytical flow liquid chromatography-mass spectrometry, microflow liquid chromatography-mass spectrometry, nanoflow liquid chromatography-mass spectrometry, and ultra-low flow capillary electrospray ionization mass spectrometry. These ensure reproducibility, reliability, and prevention of carryover between injections. The integrated software ensures correct chromatography data flow, data integrity, smooth usability, and enhanced efficiency for mass spectrometry workflows, providing precision, durability, and seamless integration with cutting-edge mass spectrometers, allowing laboratories to address challenges with speed, accuracy, and a complete solution.
What are the best practices for maintaining HPLC system performance?
Maintaining optimal HPLC system performance involves regular maintenance schedules, proper column care, and attention to mobile phase quality, sample preparation, and injection techniques. Temperature control, quality control standards, system flushes, software updates, personnel training, and effective waste management contribute to ensuring reliable and accurate chromatographic results while extending the longevity of the equipment.
For additional details, please get in touch with our life sciences expert.
Are there specific precautions for using HPLC systems with volatile samples?
When working with volatile samples in HPLC systems, it is crucial to select a compatible volatile mobile phase, use appropriate injection techniques, and maintain column oven temperature control to prevent sample evaporation and ensure effective separation. Additionally, ensuring seal integrity, employing proper sample containment methods, adjusting detector sensitivity, and observing safety precautions are essential measures to optimize the accuracy of analyses while working safely with volatile compounds.
What are the common challenges faced in HPLC system troubleshooting?
HPLC system troubleshooting commonly involves addressing challenges such as retention time drift, baseline noise, and peak shape distortion due to factors like column conditions and sample overloading. Issues like pressure fluctuations, gradient inaccuracies, and detector irregularities necessitate systematic evaluation. At the same time, meticulous maintenance and adherence to best practices are crucial for preventing and resolving common problems in chromatographic analyses.
Are there any recommended accessories for optimizing HPLC system functionality?
Recommended accessories for optimizing HPLC system functionality include an optional wash system module, various detector choices, and switching valves, providing environmentally conscious systems with a harmonious balance between sensitivity and durability. Additionally, user-friendly autosamplers with scalable injection volumes, UHPLC pumps featuring automatic purging and an integrated four-channel degasser, and HPLC columns with a unique fingertight end-fitting system for easy installation contribute to improved stability and exceptional chromatographic performance. Incorporating capillary electrophoresis and electrospray ionization in a single device enhances researchers' experiences, offering minute sample consumption and streamlined workflows for protein therapeutics characterization. The comprehensive selection of optical detectors seamlessly integrated with MS solutions ensures a wide spectrum of choices for enhanced efficiency in mass spectrometry workflows across the laboratory.
How does the HPLC system contribute to method development in research?
The HPLC system contributes to method development in research by enabling systematic optimization of parameters, including mobile phase composition and column types, facilitating selectivity studies, and assisting in sample cleanup. Additionally, it plays a crucial role in method validation, quantitative analysis, and the integration of mass spectrometry, offering researchers precise and versatile tools for developing robust analytical methods across various scientific domains.