Chromatography Columns

What are some of the different chromatography column types offered by us?

We provide an extensive array of solid supports, encompassing core-shell, organo-silica fully porous, and thermally modified fully porous particles, all available in a micro-LC format. Our LC columns, essential for separating and quantifying compounds based on their chemical properties, are available in various formats, from nano to analytical UHPLC to preparative, these column ensure compatibility with a wide range of instruments for method execution.

Our UHPLC columns allow you to dramatically enhance efficiency and achieve extraordinary resolution results in your separations on any system as a leading provider in the UHPLC field.

Our nano columns featuring integrated fittings were specifically engineered to seamlessly work with a wide range of currently available systems in liquid chromatography labs, offering a user-friendly fitting that ensures reliable connections by preventing both over-tightening and under-tightening, guaranteeing leak-free performance.

Guaranteed to be the longest-lasting preparative LC columns available, our advanced column packings and hardware come in over 40 distinctive achiral and chiral selectivities, offering enhanced performance through increased sorbent bed density while eliminating media bed collapse as a source of premature column failure.

Guard columns and cartridges often used in liquid chromatography are designed to effectively (and inexpensively) safeguard your valuable chromatography columns from the damaging effects of chemical contaminants without altering your chromatographic results are designed with the same media options as analytical columns to ensure method compatibility.

GC Columns

GC capillary columns offer attributes like low bleed, exceptional retention, heightened sensitivity, and extended longevity reducing the baseline noise, possibility of ghost peaks, and improving resolution.

Our GC coiumns are classified into 4 distinct categories based on separation needs and method compatibility: Standard, Improved Performance, High Temperature and Application Specific Columns to cover a wide range of chromatographic needs.

GC metal columns, used in our High Temperature GC Columns, involve the deposition of a uniform glass layer over the internal metal surfaces of the column for better peak shape and resolution.

What are the main types of chromatography and their differences?

Liquid chromatography: Utilizes a liquid mobile phase flowing through a stationary phase-coated solid support to separate compounds based on their interactions with the stationary phase.

Gas chromatography: Separates volatile compounds using a stationary phase-coated solid support and an inert gas as the mobile phase.

Liquid chromatography subtypes based on diverse techniques:

Flash chromatography: A large column filled with silica media for higher flow rates to move the liquid mobile phases through the flash chromatography columnfor a quicker purification of compounds.

HPLC (High Performance Liquid Chromatography) Employs high-pressure compatibilite columns, particles and pressure limits to separate components in a liquid mixture which can then be detected or analytzed further by diverse detection modes.

Large-scale column chromatography: Involves separating and isolating substantial quantities of target compounds from mixtures.

Preparative HPLC: Utilizes HPLCto separate or purify large quantities of mixtures (up to samples volumes in kilograms) for further use. This technique uses larger Internal diameter (ID) columns compared to analytical columns to increase flow rate.

UHPLC (Ultra High Performance Liquid Chromatography): A rapid and high-resolution variant of HPLC that enhances separation efficiency and speed with smaller particles that are able to obtain higher pressure to increase sensitivity of analysis

Liquid chromatography subtypes based on diverse separation mode

Chiral chromatography: Separates enantiomers based on their different interactions with a chiral column chromatography stationary phase.

Size exclusion chromatography: Separates molecules based on size using porous particles to allow smaller molecules to enter the pores, leading to their slower elution.

HILIC: Separates polar compounds using a hydrophilic stationary phase and a more organic mobile phase.

Ion exchange chromatography: Separates ions based on their charge by using a charged stationary phase that attracts or repels ions.

Ion exclusion chromatography: Separates ions by their size and charge using a stationary phase that excludes ions from its pores.

Normal phase chromatography: Uses a polar stationary phase and a non-polar mobile phase to separate compounds based on their polarity.

Reversed phase chromatography: Employs a non-polar stationary phase and a polar column chromatography mobile phase to separate compounds based on their hydrophobicity.

Gas chromatography subtypes

For specialty applications: Provide specific selectivity for unique applications, such as determining blood alcohols.

For high-temperature applications: Used for high-molecular-weight compounds that necessitate temperatures exceeding 360 °C.

For improved performance: For applications demanding stability, performance, inertness, reproducibility, and low bleed.

For standard analysis: Feature a diverse spectrum of polarities, spanning low, medium, and high polar phases.

What factors should be considered in column chromatography?

Column chromatography selection depends on the right stationary and mobile phase composition for your unique target analytes separation needs, along with flow rate, elution order prediction, and safety considerations. Column dimensions, detection modes, chromatography column maintenance cost, the optimal design of a reservoir system for consistent sample flow, and temperature further contribute to the efficiency of the process.