ZenoTOF 7600 System for Mass Spectrometry

Dive deeper into the biomarker landscape. Zeno SWATH DIA marks a significant step-change in data-independent acquisition delivering a high depth of coverage, particularly on low abundance species, quickly and robustly.

The Zeno trap in combination with SWATH Acquisition enables significant sensitivity gains through the use of Zeno trap enabling researchers to routinely quantify up to twice the number of plasma proteins than previously possible.

With sample loads as little as 10 ng and runtimes shortened to as little as 5 mins, large-scale biomarker studies can run as routine projects that are achieved in a matter of weeks, without compromising the depth of proteome coverage that can be obtained.

Zeno SWATH DIA allows the highest level of depth in your data in the shortest amount of time compared to traditional SWATH DIA.

Electron Activated Dissociation (EAD)

EAD allows for a range of free electron based fragmentation mechanisms within one device. The ability to tune electron kinetic energy within an EAD experiment extends the utility of the approach to all molecules types, from singly charged small molecules to large multiply charged proteins.

• Protein based therapeutics come with a rich assortment of varying structures. Those modifications exist in complex heterogeneous mixtures.
• CID has been used extensively to determine the structure of biomolecules but full characterization can be difficult without further sample processing e.g. digestions.
• Low energy EAD fragmentation enables characterization of intact biomolecules via top down approaches allowing researchers to rapidly identify and confirm key structural attributes.
• Qualitative flexibility combined with quantitative power

• When tuned to higher kinetic energies, EAD has the capability to fragment post-translationally modified peptides such as phosphopeptides and glycopeptides whilst retaining critical MS/MS information for both identification and localisation of PTMs. Unlike other electron-based fragmentation techniques, this can be achieved in conjunction with fast scan speeds and in a reproducible, consistent manner.
• The EAD cell brings fresh insight and completes the picture for biomolecule characterization. EAD, fragmentation of large multiply-charged produces those differential C & Z ions to garner those critical insights.
• A new electron activated dissociation (EAD) approach for comprehensive glycopeptide analysis of therapeutic proteins
• Tuneable electron activated dissociation (EAD) MS/MS to preserve particularly labile post translational modifications

• CID fragmentation of small molecules can produce limited and/ or non-specific MS/MS information, giving rise to challenges in identification or lack of specificity in the development of quantitative assays.
• High-energy EAD fragmentation can produce highly specific MS/MS information on small molecules such as endogenous metabolites or the products of drug metabolism. This enables confident, unambiguous identification and highly specific MS/MS-based quantification.
• Complete structural elucidation of lipids in a single experiment using Electron-Activated Dissociation (EAD)

ZENO TRAP

• The next era of sensitivity for accurate mass
• Ions are accumulated in the Zeno trap before being pulsed rapidly into the TOF, meaning we can detect up to 20x more ions.
• Consequently, each TOF experiment contains more useful MS/MS information, particularly on lower abundance species that were previously undetectable, introducing our customers to a new sensitivity revolution.

Benefits of ZenoTOF 7600 system

Rare quantifiable data becomes your everyday

• Zeno SWATH DIA - New depth of rare data.
• Zeno EAD/ CID - Fresh perspective and certainty.
• Zeno MRMHR - Fresh perspective and certainty.

Zeno Enabled data landscape

Zeno SWATH DIA

Zeno trap pulsing on demand gives the ability to detect lower abundance ions with those in abundance at the same time driving the limits of quantification achievable with accurate mass. Zeno MRM

Zeno DDA (CID/EAD)

EAD technology allows you to fine tune the electron energy, accessing multiple fragmentation mechanisms that can be applied to large molecules, peptides, lipids and small molecules.

• 40% more proteins identified using Zeno MS/MS
• Characterization of lipids in a single experiment
• Comprehensive coverage and localized glycans in a single run

Zeno trap pulsing on demand gives the ability to detect lower abundance ions with those in abundance at the same time driving the limits of quantification achievable with accurate mass. Zeno MRM

SCIEX OS is built on a foundation of powerful algorithms and automation that enable efficient data interpretation, at scale, to the level needed for clinical relevance.

It's remarkable quantitative useability makes collaboration easy enabling researchers across labs, countries and continents to share insights and produce meaningful impact.

SCIEX OS is powering the Zeno revolution.

Flow rate

Turbo V source

• Refined source design that limits contamination for maximized uptimes.
• High sensitivity across 5 µL/min - 3 mL/min
• Compound class coverage
• APCI and ESI modes

Optiflow source

• Robustness and simplicity for high-sensitivity microflow workflows.
• Intelligent probe and electrode design
• Eliminates manual adjustment
• Minimized source optimization for the 1 -200 µL/min workflows.

Optiflow Turbo V source

• Allows the user to switch to nanoflow regimes for the highest sensitivity.
• Plug-and-play nanoflow capability
• Integrated column oven
• Nanoflow probe and electrodes