Primary Screening Assays
To initiate the screening process, compounds should be incubated with either reagents or cells. The duration of incubation varies depending on the specific assay being conducted. For instance, qPCR-based assays may require only milliseconds for binding, whereas cancer phenotype assays may require several days to achieve adequate incubation. Automate the process with a Beckman Coulter Life Sciences Biomek i-Series Automated Liquid Handler. If assays are miniaturized, acoustically dispense reagents with a Beckman Coulter Life Sciences Echo Acoustic Liquid Handler. Ensure incubation times are appropriate for screening methods and aims. Automate the process with a Beckman Coulter Life Sciences Biomek i-Series Automated Liquid Handler. If assays are miniaturized, acoustically dispense reagents with a Beckman Coulter Life Sciences Echo Acoustic Liquid Handler. Ensure incubation times are appropriate for screening methods and aims. Screening readouts will require different instruments for detection, such as plate readers, high-content imagers, flow cytometers, or even high-throughput mass spectrometers. Readouts may include a fluorescent response, ATP assay, live/dead cell imaging, mass spectra, etc. As readouts are recorded, automatically calculate plate-based QC metrics, analyze assay readouts — including for biochemical, cell-based and other assays, regardless of throughput — and select top hits using the assay analysis capabilities found in the Genedata Biopharma Platform.
The life sciences companies of Danaher offer the following analytical and data management solutions to help with your screening:
- IDBS Polar Biopharma lifecycle management software
- Molecular Devices ImageXPress HT.ai High Content Imaging System
- Molecular Devices FLIPR Penta High Throughput Cellular Screening System
- Beckman Coulter Life Sciences Vi-CELL BLU Cell Viability Analyzer
- Beckman Coulter Life Sciences CytoFLEX Flow Cytometer
- Molecular Devices SpectraMax iD5 Plate Reader
- SCIEX Echo MS System
- Genedata Biopharma Platform software