Screening Process Workflow Solutions - One lab, three paths to faster outcomes
LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Compound Library Preparation
Create liquid stocks of compounds for use, dissolving powdered molecules in aqueous DMSO or creating plates containing biologics for screen. Store stock solutions using care to prevent potential degradation of compounds.
DIGITAL SOLUTIONS
Library Maintenance
Track compound usage and integrity over time, maintaining usable stocks of compounds for screening.
LABORATORY AUTOMATION
Reagent Plate Creation
Dispense small volumes from stock solutions onto working reagent plates for use in screening protocols. Volumes on reagent plates are used as working stock and discarded to prevent contamination of compound stocks.
LABORATORY AUTOMATION
Assay Plate Creation
Create screening plates by dispensing cells into individual wells for testing effects of different compounds. Cell types used and molecules screened will inform analysis.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Primary Screening Assays
Perform primary screening for the desired effect with a fluorescent response, live/dead imaging or other readouts. Hits that have an effect are taken forward.
LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Cherry Picking
Select successful hits to bring forward to primary screens. Consolidate hits across working reagent stocks onto new assay plates.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Secondary Screening Assays
Iterate on screening hits using more complex or orthogonal assays, such as performing dose-response assays in vitro or studying the effects on gene expression. Secondary screening assays will depend on the types of molecules being screened.
DIGITAL SOLUTIONS
Data Analysis
Study the strength of effect and efficacy to generate leads and narrow the funnel of potential hits. Statistical analyses will become more complex, such as multi-parametric analyses.
LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Secondary Cherry Picking
Select successful hits to bring forward to secondary screens. Consolidate hits across working reagent stocks onto new assay plates.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Hit Profiling / Lead Optimization
Screen compounds using more complex methods to determine pharmacokinetics and toxicity to select best candidate compounds for in vivo studies.
DIGITAL SOLUTIONS
Hit Profiling / Lead Optimization Data Analysis
Study compound kinetics, mechanism of action and safety profiles. Compounds that have progressed through multiple rounds of screening continue to in vivo studies.
