Gene Modification and Analysis in CRISPR-Mediated Gene Therapy
The delivery of gRNA and Cas reagents can vary depending on the recipient cell line. Automate your chemical transfection process and ensure consistency with a Beckman Coulter Life Sciences Biomek i-Series Integrated Workstation.
Post-modification, assess cell counts and viability using a Beckman Coulter Life Sciences Vi-CELL BLU Cell Viability Analyzer. More in-depth analyses, such as mitotic analyses and fluorescent imaging, can be performed on a Leica Microsystems STELLARIS Confocal Microscope Platform or automated on a Leica Microsystems Mica Microhub. Automatically capture and analyze these data, including imaging and other fluorescent assays, using the Genedata Biopharma Platform. With the software, register every component of a CRISPR-mediated gene therapy, including Cas and sgRNA-encoding plasmids, delivery systems (e.g., LNPs, viral vectors) and purified final products.
Collect and track experiment and instrument data with the IDBS Polar Biopharma Lifecycle Management software.