CRISPR-Mediated Gene Therapy Process Steps | Danaher Life Sciences

CRISPR-Mediated Gene Therapy- One lab, three paths to faster outcomes

All

Leverage the extensive portfolios of our Life Sciences companies and accelerate Preclinical and Clinical Development for Gene Therapies.

Laboratory Automation

Increase workflow reproducibility and standardization with cutting-edge tools.

Analytical Tools

Gain critical insights faster with scalable solutions.

Digital Solutions

Enterprise-level workflow management, data handling and analyses.

1

LABORATORY AUTOMATION | DIGITAL SOLUTIONS | ANALYTICAL TOOLS

Preliminary Screening

Select the target, develop the proper gene editing strategy, and screen libraries to select the gRNA.

2

LABORATORY AUTOMATION | DIGITAL SOLUTIONS | ANALYTICAL TOOLS

Isolate & Enrich

Obtain patient samples to isolate and enrich the desired cell population.

3

LABORATORY AUTOMATION | DIGITAL SOLUTIONS | ANALYTICAL TOOLS

Gene Modification and Analysis

Edit the target gene via a CRISPR-Cas approach and analyze for expected effect.

4

ANALYTICAL TOOLS | DIGITAL SOLUTIONS

Expansion

Expand CRISPR-edited cells for downstream characterization.

5

LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS

Characterization and Optimization

Characterize molecular consequences of CRISPR edits and optimize as needed.

6

ANALYTICAL TOOLS | DIGITAL SOLUTIONS

Functional Assessment

In vitro and in vivo functional activity assessment is performed to inform on risk.

7

LABORATORY AUTOMATION | DIGITAL SOLUTIONS

Off-Target Analysis

Chromosomal, sequencing and in silico analyses help uncover and understand off-target effects.

8

DIGITAL SOLUTIONS

Regulatory Interaction

Prepare data and reports for regulatory filings in support of the CRISPR-mediated gene therapy.