Analysis of Off-Target Effects in CRISPR-Edited Cells
Use next-generation sequencing (NGS) to detect and quantify on-target and off-target editing events in CRISPR-edited cells at predicted or experimentally nominated genomic loci. Prepare targeted NGS libraries using using Beckman Coulter Life Sciences extraction reagents, IDT rhAmpSeq CRISPR Analysis System, and a Beckman Coulter Life Sciences Biomek i-Series Automated Workstation. With the Genedata Biopharma platform, automate sequencing data analysis with workflows designed to characterize potential off-target sites and other genome editing outcomes.