Functional Assessment of CRISPR-Edited Cells
CRISPR-edited cells are assessed for proper functional activity; safety and efficacy are paramount. Cells must be evaluated for potency and toxicity before being delivered to a patient. Readouts, including a fluorescent response, ATP assay, live/dead imaging of cells, or other image-measured responses, are performed with a Molecular Devices multi-mode plate reader with GxP-compliant software.
Cell-based assays are leveraged for functional assessments and require the ability for live-cell and time-lapse imaging. A Leica Microsystems STELLARIS Confocal Microscope Platform or Leica Microsystems Mica Microhub fulfills these requirements for imaging and cell-based functional assays.