Characterization and Optimization of CRISPR Edits
Determining if CRISPR modifications are integrated at the correct loci with no off-target effects is critical. Next-generation sequencing (NGS) is a popular assessment method. IDT's award-winning rhAmpSeq CRISPR Analysis System is specifically designed to analyze genome editing results. Automate the DNA extraction and NGS library prep protocols on a Beckman Coulter Life Sciences Biomek i-Series Automated Workstation. After sequencing, analyze data using the Genedata Selector software to characterize integration efficiency and potential off-target sites.
Determining the consequences of genome editing on cell phenotype and protein expression is essential. Ensure cells express the gene-of-interest (GOI) as intended with correct post-translational modifications. Optimize CRISPR-Cas modifications and protein expression if required. The SCIEX ZenoTOF 7600 System can unlock insights for protein and peptide modifications. Streamline mass spectrometry data handling and analyses using the Genedata Expressionist software.
Flow cytometry can also analyze protein expression from CRISPR-edited cells. The Beckman Coulter Life Sciences CytoFLEX Flow Cytometer enables preclinical lab analysis. The Beckman Coulter Life Sciences CellMek SPS System can automate clinical flow cytometry sample preparation. Flow cytometry data analysis uses Beckman Coulter Life Science’s Cytobankand Beckman Coulter Life Science’s Kaluza C software
Optimization of gRNA sequences and assay readouts will require the ability to process and manage large datasets. Automatically capture, store, recall and format data with the IDBS Polar Biopharma Lifecycle Management software. Data traceability is critical, as regulatory audits require secure recordkeeping documenting all processes and data.