Characterization and Optimization of CRISPR Edits
Determining if CRISPR modifications are integrated at the correct loci with no off-target effects is critical. Next-generation sequencing (NGS) is a popular assessment method. IDT's award-winning rhAmpSeq CRISPR Analysis System is specifically designed to analyze genome editing results. Automate the DNA extraction and NGS library prep protocols on a Beckman Coulter Life Sciences Biomek i-Series Automated Workstation. Use the Genedata Biopharma Platform to automate analysis of sequencing data with purpose-built workflows for characterizing integration efficiency and potential off-target sites.
Determining the consequences of genome editing on cell phenotype and protein expression is essential. Ensure cells express the gene-of-interest (GOI) as intended with correct post-translational modifications. Optimize CRISPR-Cas modifications and protein expression if required. The SCIEX ZenoTOF 7600 System can unlock insights for protein and peptide modifications. Streamline characterization, including detection of post-translational modifications, sequence variants and GOI verification, using built-in mass spectrometry and NGS analysis workflows in the Genedata Biopharma Platform.
Flow cytometry can also analyze protein expression from CRISPR-edited cells. The Beckman Coulter Life Sciences CytoFLEX Flow Cytometer enables preclinical lab analysis. The Beckman Coulter Life Sciences CellMek SPS System can automate clinical flow cytometry sample preparation. Flow cytometry data analysis uses Beckman Coulter Life Science’s Cytobankand Kaluza C softwareAutomate flow cytometry data analysis using the Genedata Biopharma Platform.
Optimization of gRNA sequences and assay readouts will require the ability to process and manage large datasets. Automatically capture, store, recall and format data with the IDBS Polar Biopharma Lifecycle Management software. Data traceability is crucial, as regulatory audits necessitate secure record-keeping that documents all processes and data. The Genedata Biopharma Platform serves as a central hub for data capture, analysis and management, allowing registration of all CRISPR modifications and associated characterization results.