Isolation & Enrichment in CRISPR-Mediated Gene Therapy
Understand and enumerate your starting sample cell types, including CD34+ populations, using a Beckman Coulter Life Sciences Aquios CL that automates sample prep, flow cytometry and analysis. Isolation can be performed with a Beckman Coulter Life Sciences CytoFLEX SRT Benchtop Cell Sorter by sorting individual cells into wells. A Beckman Coulter Life Sciences Avanti JXN High-Performance Centrifuge is used to concentrate cells. Assess post-concentration viability using a Beckman Coulter Life Sciences Vi-CELL BLU Cell Viability Analyzer.
Track experiment and instrument data, including sorted cells selected for enrichment in IDBS Polar Biopharma Lifecycle Management software.