2D Preculture
Starting with a monolayer culture is crucial to expand cell populations and obtain necessary cell numbers for organoid development. Organoid culture and development can be completely automated using a Molecular Devices CellXpress.ai Automated Cell Culture System
If you are looking to integrate fit-for-purpose solutions with your current laboratory hardware, a Beckman Coulter Life Sciences Biomek i-Series Automated Workstation can prepare plates and a Molecular Devices ImageXpress Confocal HT.ai can monitor monolayer cultures to help standardize your process. Cytiva cell culture media can help you obtain and maintain a healthy cell population.
When your 2D monolayer is ready to move forward with 3D culture, remove cells from the dish and centrifuge with a Beckman Coulter Life Sciences Allegra V15R Benchtop Centrifuge followed by cell counting and viability information on the Beckman Coulter Life Sciences Vi-CELL BLU Cell Viability Analyzer.