Maintenance & Passaging
Organoid culture duration varies from slightly longer than a week to a few months. Routine maintenance of the culture, like changing cell culture media, is mundane and pulls researchers away from more important tasks. A Beckman Coulter Life Sciences Biomek i-Series Automated Workstation can cover routine media exchanges and feeding schedules with ease. The Molecular Devices ImageXpress Confocal HT.ai and Molecular Devices IN Carta Image Analysis software are tools to monitor organoid growth and architecture over time.
Depending on the organoid type and duration of culture, rapid growth may necessitate splitting cultures. If dissociating your organoids to single cells prior to replating, rely on the Beckman Coulter Life Sciences Allegra V15R Benchtop Centrifuge and Beckman Coulter Life Sciences Vi-CELL BLU Cell Viability Analyzer to preserve a healthy cell population when passaging.