Organoid Development Workflow Solutions - One lab, three paths to faster outcomes
SERVICES
Select Starting Material
Select the proper starting cell type based on the disease model or drug screening assays of interest. Secondary considerations should include required media, reagents, and consumables.
LABORATORY AUTOMATION
Tissue Dissociation
If using a patient biopsy sample or solid tissue as starting material, dissociate to ensure populations of single cells. Proceed with caution as mechanical and enzymatic methods can unintentionally reduce viable cell counts.
LABORATORY AUTOMATION
Cell Washing & Preparation
Prepare for 2D monolayer culture by washing and centrifuging your starting cell population(s) to remove residual materials. Count cells to ensure proper seeding densities.
LABORATORY AUTOMATION | ANALYTICAL TOOLS
2D Preculture
Seed cells onto plates or vessels at the proper density for optimal growth. It is important to select proper cell culture media and determine whether cells require a scaffold matrix to attach.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Organoid Culture
Plate your mixture of cells and scaffold matrix in desirable quantities. 3D organoid architecture will start to form over the next days to weeks.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Maintenance & Passaging
Monitor and maintain organoid culture conditions to ensure desired growth and physiological architecture is obtained for downstream assays. Passaging may be required and could include reduction of organoid density or dissociation to single cells prior to replating.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Assays & Analyses
Dose organoids with drug compound libraries or biologics of interest. Obtain readouts from primary and secondary assays to select hits.
DIGITAL SOLUTIONS
Data Aggregation
Aggregate data to determine hits from drug or biologic screens. Ranking could be based on phenotypic changes, gene expression, toxicity, metabolic readouts or protein production.
