Plasmid Analysis for AAV Vectors - Automation, Analytical and Digital Solutions
It is important to analyze plasmids for critical structural elements that may have an impact on the upstream and downstream processing of AAVs. Several parameters and testing methods are used to achieve this, including residual host DNA/RNA, endotoxin, mycoplasma, sterility, purity and sequencing. Additionally, determining plasmid titers is essential.
Molecular Devices SpectraMax plate readers provide imaging readouts for various QC assays such as DNA quantification, sample sterility, endotoxin and mycoplasma screening. Molecular Devices and Beckman Coulter Life Sciences offer integrated workflow solutions to cover assay steps.
It is crucial to perform structural quality assessments that check for coiling, perform fragment analysis, and detect isoforms and host cell DNA/RNA. The SCIEX PA800 Plus system uses capillary electrophoresis with integrated analysis software to uncover these parameters.
NGS library preparation can be fully automated using the Beckman Coulter Life Sciences Biomek i-Series Automated Workstation and the Beckman Coulter Life Sciences Echo Acoustic Liquid Handler, which sustainably minimizes reagent volume consumption. Genedata's Selector software can analyze and share your findings across the organization.
Ensure all plasmid analysis assays and findings are documented in the IDBS Polar Biopharma Lifecycle Management platform for streamlined reporting.