AAV pDNA Workflow Solutions - One lab, three paths to faster outcomes
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DIGITAL SOLUTIONS
pDNA Synthesis
Design and synthesize pDNA sequences for optimal AAV vector packaging and expression.
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LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Gene Assembly
Amplify and assemble plasmid components such as vector backbone, transcription regulation components and the gene of interest.
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ANALYTICAL TOOLS | LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Plasmid QC
It is crucial to ensure the plasmid's integrity through sequencing or capillary electrophoresis to confirm the presence of critical genes and structural elements.
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ANALYTICAL TOOLS | LABORATORY AUTOMATION
Transformation & Recovery
E. coli cells are transformed and allowed to recover in specialty media.
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LABORATORY AUTOMATION | ANALYTICAL TOOLS
Colony Picking
Automate colony picking based on selection criteria. Colonies should be screened before expansion.
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ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Fermentation / Expansion
Transformed colonies are selected and expanded in a fermenter for adequate plasmid quantity needed for testing, banking and cGMP manufacturing.
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ANALYTICAL TOOLS | LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Plasmid Extraction & Purification
To conduct quality control checks, plasmids are extracted and purified from lysed E. coli cells.
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LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Plasmid Analysis
Analyze plasmids for mutations, impurities and contaminants. Capture assay data and generate batch reports before cell banking.
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DIGITAL SOLUTIONS
Cell Banking
Freeze and track AAV plasmid cell banks that will be used for scale-up and cGMP manufacturing.