AAV pDNA Workflow Solutions - One lab, three paths to faster outcomes
DIGITAL SOLUTIONS
pDNA Synthesis
Design and synthesize pDNA sequences for optimal AAV vector packaging and expression.
LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Gene Assembly
Amplify and assemble plasmid components such as vector backbone, transcription regulation components and the gene of interest.
ANALYTICAL TOOLS | LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Plasmid QC
It is crucial to ensure the plasmid's integrity through sequencing or capillary electrophoresis to confirm the presence of critical genes and structural elements.
ANALYTICAL TOOLS | LABORATORY AUTOMATION
Transformation & Recovery
E. coli cells are transformed and allowed to recover in specialty media.
LABORATORY AUTOMATION | ANALYTICAL TOOLS
Colony Picking
Automate colony picking based on selection criteria. Colonies should be screened before expansion.
ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Fermentation / Expansion
Transformed colonies are selected and expanded in a fermenter for adequate plasmid quantity needed for testing, banking and cGMP manufacturing.
ANALYTICAL TOOLS | LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Plasmid Extraction & Purification
To conduct quality control checks, plasmids are extracted and purified from lysed E. coli cells.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Plasmid Analysis
Analyze plasmids for mutations, impurities and contaminants. Capture assay data and generate batch reports before cell banking.
DIGITAL SOLUTIONS
Cell Banking
Freeze and track AAV plasmid cell banks that will be used for scale-up and cGMP manufacturing.
