pDNA Template Testing (Sequence Verification; Residual Protein; Reagent Testing)
Testing pDNA for purity, sequence fidelity and residual protein contaminants are important for mRNA transcription success. Analytical testing with SCIEX Biophase 8800 Multi-capillary Electrophoresis system for purity, size and DNA coiling will help to verify consistent in vitro transcription. Study optical density and absorbance on a Molecular Devices SpectraMax plate reader to understand concentrations and purity. pDNA is an important reagent, subject to the same rigorous quality controls and standards as chemical reagents. With the Genedata Biopharma Platform, capture, analyze and manage all pDNA testing and critical quality attribute (CQA) data in a GMP-compliant manner. Covering chromatography, capillary electrophoresis, mass spectrometry, sequencing, qPCR, absorbance assays and more, the software automates and harmonizes analysis, linking all results to their associated purification batches.