pDNA Template Testing (Sequence Verification; Residual Protein; Reagent Testing)
Testing pDNA for purity, sequence fidelity and residual protein contaminants are important for mRNA transcription success. Analytical testing with SCIEX Biophase 8800 Multi-capillary Electrophoresis System for purity, size and DNA coiling will help to verify consistent in vitro transcription. Study optical density and absorbance on a Molecular Devices SpectraMax plate reader to understand concentrations and purity. Consider pDNA an important reagent, subject to the same rigorous quality controls and standards as chemical reagents and choose the best candidates with the Genedata Expressionist Software.