Overview of In Vitro Transcription
In vitro transcription creates mRNA oligonucleotides from pDNA templates in a one-pot reaction precisely controlled for maximum efficiency. Other enzymatic steps can be performed at this time if desired, such as 5’ capping. Additional enzymatic processing steps may be added to the “one-pot” reaction but increase complexity and quality control requirements. Aldevron can fulfill your IVT enzyme needs and offer comprehensive testing and quality oversight to match your scale. Data ecosystem management with IDBS Polar Biopharma Lifecycle Management platform can enable process standardization by breaking down data silos.
Genedata Bioprocess increases the efficiency of the end-to-end mRNA development workflow, covering all plasmid production steps (fermentation, harvest, prep, linearization, and filtration), in vitro transcription, and necessary purification and modification operations. It includes formulation screening to identify optimal delivery systems, including lipid nanoparticle (LNP) composition tracking. It facilitates workflow automation, barcoding, and high-throughput processes, capturing all data in a structured format providing a robust data foundation for predictive modeling and AI/ML.