SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers
Tunable wavelength selection, onboard injectors, and optional environmental controls to support cell-based & microbial assays
Designed for Today’s Assays and Tomorrow’s Breakthroughs-flexible detection that evolves with your science.
Multi-Mode Microplate Readers
The SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers deliver flexible, scalable solutions for absorbance, fluorescence, and luminescence assays—with advanced capabilities for evolving research needs.
The iD3s reader offers a streamlined, affordable platform with a tunable monochromator and temperature control, while the iD5e reader adds enhanced detection modes including time-resolved fluorescence (TRF) and tunable fluorescence polarization (FP) and can be expanded to include TR-FRET, HTRF, BRET, and western blot. Both readers support onboard fluidics with optional dual injectors.
Environmental Control for Live-Cell Assays
For live-cell assays and microbial growth experiments, environmental control for CO₂/O₂ regulation and advanced shaking are available to maintain ideal growth conditions.
Data acquisition and analysis are driven by SoftMax® Pro Software—the most cited microplate reader software in the industry.
Supports Diverse Assay Needs
Perform ELISA, nucleic acid and protein quantitation, cell viability, microbial assays, western blot, and more.
Simplified Setup & Analysis
Over 200 preconfigured protocols and intuitive setup tools help streamline data acquisition and analysis—backed by the industry’s most cited microplate reader software.
Fast, Consistent Mixing for High-Performance Flash Assays
Dual injectors available for fast kinetic assays with SmartInject® Technology, ensuring complete reagent mixing in every well for high precision with 10 µL dead volume.
Superior Hybrid Optical System
The high-efficiency monochromator and filter-based optical system, combined with an ultra-cooled photomultiplier tube (PMT), minimizes noise while increasing sensitivity, selectivity, and dynamic range.
Automatic Filter Identification
Automatically identify filter sets using NFC functionality, simplifying workflows and ensuring accuracy and reliability.
Read Height Optimization (Z Height)
Z-height optimization automatically adjusts the detection height to maximize sensitivity and signal quality—while minimizing sample volume—ensuring reliable results even in low-volume or precious samples.
Personalized Touchscreen Control
The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.
Live Cell Assays
Advanced shaking and user-installable gas mixer (CO₂/O₂) that support ideal environments for cell-based and microbial growth experiments are available as optional upgrades.
Flexible Temperature Control
Precise and intuitive temperature regulation—from 5°C above ambient conditions up to 66°C—empowering your lab to perform a wide range of temperature-sensitive assays with confidence.
Microvolume & Cuvette Options
The SpectraDrop Micro-Volume Microplate for low-volume (2 to 4 μL) and SpectraCuvette™ Adapter Plate for cuvette-based applications eliminate the need for additional instrumentation.
Western Blot Capable
The optional enhanced time-resolved fluorescence (TRF) detection module is optimal for quantitating as little as femtogram protein samples, providing improved sensitivity, extended signal stability, and versatility to support a wide range of applications.
| Item Height Range (Metric cm) | 40.1 cm |
| Item Width Range (Metric cm) | 53.2 cm |
| Microplate Reader Type | Multi-Mode Reader |
| Detection/Read Mode(s) | Fluorescence Intensity (Top/Bottom Read), Absorbance, Luminescence (Top/Bottom Read), Fluorescence Polarization (Top Read), Homogenous Time-Resolved Fluorescence (Top/Bottom Read), Time-Resolved Fluorescence (Top/Bottom Read), Homogenous Time-Resolved Fluorescence |
| Detection/Read Type(s) | Endpoint, Spectrum Scan, Well Scan |
| Absorbance Read Time Range (sec) | 28 sec |
| Fluorescence Intensity Read Time Range (sec) | 21 sec |
| Luminescence Read Time Range (sec) | 21 sec |
| Fluorescence Polarization Read Time Range (sec) | 35 sec |
| Time-Resolved Fluorescence Read Time Range (sec) | 25 |
| Minimum Absorbance Wavelength Range (Metric nm) | 230 nm |
| Maximum Absorbance Wavelength Range (Metric nm) | 1000 nm |
| Minimum Fluorescence Intensity Wavelength Range (Metric nm) | 250 nm |
| Maximum Fluorescence Intensity Wavelength Range (Metric nm) | 850 nm |
| Minimum Luminescence Wavelength Range (Metric nm) | 300 nm |
| Maximum Luminescence Wavelength Range (Metric nm) | 850 nm |
| Minimum Time-Resolved Fluorescence Wavelength Range (Metric nm) | 350 nm |
| Maximum Time-Resolved Fluorescence Wavelength Range (Metric nm) | 750 nm |
| Minimum Fluorescence Polarization Wavelength Range (Metric nm) | 300 nm |
| Maximum Fluorescence Polarization Wavelength Range (Metric nm) | 750 nm |
| Fluorescence Intensity Sensitivity Top Read Optimization | Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well |
| Luminescence Sensitivity Top Read Optimization | 2 pM ATP 96 well, 4 pM ATP 384 well |
| Time-Resolved Fluorescence Sensitivity Top Read Optimization | TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well |
| Fluorescence Polarization Sensitivity Top Read Optimization | Mono: 2 mP @ 10nM 96 well, 2 mP @ 10nM 384 well Filters: 1 mP @ 1 nM 96 well, 2 mP @ 1 nM 384 well |
| Microplate Photometric Accuracy | < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV |
| Photometric Precision | < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV |
| Microplate Reader Detector(s) | Photomultiplier Tube, Silicon Photodiode |
| Light Source(s) | High Power Xenon Flash Lamp |
| Microplate Size Compatibility | 6 well plate, 12 well plate, 24 well plate, 48 well plate, 96 well plate, 384 well plate |
| Microplate Shaking Mode(s) | Linear, Orbital, Double Orbital |
| Wavelength Selection | Monochromator tunable in 1 nm increments, Filters |
| Microplate Reader Validation Tool(s) | SoftMax® Pro GxP Software, Absorbance Validation Plate, Fluorescence Validation Plate, Luminescence Validation Plate, Multi-Mode Validation Plate |
| Includes Validation Tools | Yes |
| Includes Cuvette Port | Optional |
| Includes Imaging Capability | Optional |
| Includes Stand-Alone Touchscreen Operation | Yes |
| Includes Western Blot Capability | Yes |
| Robotics/Automation Compatible | Yes |
| Includes Temperature Control Capability | Yes |
| Minimum Temperature Control Range Added to Ambient Temperature (Metric °C) | 5 °C |
| Maximum Temperature Control Range (Metric °C) | 66 °C |
| Item Depth Range (Metric cm) | 59.8 cm |