One lab, three paths to faster outcomes
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DIGITAL SOLUTIONS
pDNA Component Design
Design plasmids in silico, selecting genes and oligonucleotide fragments for assembling plasmid libraries. Plan for assembly in-house or source entirely from service providers.
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DIGITAL SOLUTIONS
pDNA Synthesis
DNA fragments are created with the necessary structural elements for assembly. Service providers can ensure pDNA scalability from development to cGMP-grade products.
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LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Plasmid Assembly
Assemble plasmid libraries from genes of interest and fragments. Diverse libraries maximize hit discovery.
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LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Transformation
Facilitate uptake of plasmids into bacterial cells for expression.
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LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Cell Recovery
Incubate bacteria in a medium to recover from transformation stress. Optimize recovery times to improve transformation success and plasmid production.
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LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Colony Picking
Plate cultures on a selective medium, screening for colonies that are expressing pDNA. Pick colonies into a liquid culture to bulk ferment plasmids.
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LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Cell Growth
Scale up culture volumes. Optimize growth parameters to maximize plasmid yields.
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DIGITAL SOLUTIONS
Concentration
Remove cell culture waste to isolate and concentrate biomass. Define protocols that are scalable to large production volumes.
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LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
pDNA Purification
Chemically or mechanically lyse bacterial cells and purify plasmid DNA. Determine concentrations and best methods for increasing yields.
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ANALYTICAL TOOLS | DIGITAL SOLUTIONS
QC and Banking
Determine plasmid DNA integrity, structure, and fidelity. Analytical solutions established during development must scale to larger volumes and cGMP production environments.