One lab, three paths to faster outcomes
DIGITAL SOLUTIONS
pDNA Component Design
Design plasmids in silico, selecting genes and oligonucleotide fragments for assembling plasmid libraries. Plan for assembly in-house or source entirely from service providers.
DIGITAL SOLUTIONS
pDNA Synthesis
DNA fragments are created with the necessary structural elements for assembly. Service providers can ensure pDNA scalability from development to cGMP-grade products.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Plasmid Assembly
Assemble plasmid libraries from genes of interest and fragments. Diverse libraries maximize hit discovery.
LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Transformation
Facilitate uptake of plasmids into bacterial cells for expression.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Cell Recovery
Incubate bacteria in a medium to recover from transformation stress. Optimize recovery times to improve transformation success and plasmid production.
LABORATORY AUTOMATION | DIGITAL SOLUTIONS
Colony Picking
Plate cultures on a selective medium, screening for colonies that are expressing pDNA. Pick colonies into a liquid culture to bulk ferment plasmids.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
Cell Growth
Scale up culture volumes. Optimize growth parameters to maximize plasmid yields.
DIGITAL SOLUTIONS
Concentration
Remove cell culture waste to isolate and concentrate biomass. Define protocols that are scalable to large production volumes.
LABORATORY AUTOMATION | ANALYTICAL TOOLS | DIGITAL SOLUTIONS
pDNA Purification
Chemically or mechanically lyse bacterial cells and purify plasmid DNA. Determine concentrations and best methods for increasing yields.
ANALYTICAL TOOLS | DIGITAL SOLUTIONS
QC and Banking
Determine plasmid DNA integrity, structure, and fidelity. Analytical solutions established during development must scale to larger volumes and cGMP production environments.
