Plasmid Assembly
Plasmid backbones, fragments and genes of interest are ligated together. DNA fragments may require restriction enzyme digestion to facilitate Gibson or Golden Gate assemblies. Before assembling, check sequence length and shorten run time lengths, running CE samples in parallel using a SCIEX Biophase 8800 Multi-capillary Electrophoresis System. Quickly array different combinations of plasmid DNA to create a diverse library for expression trials by using a Beckman Coulter Life Sciences Echo Acoustic Liquid Handler. Track plasmids from assembly using IDBS Polar Biopharma Lifecycle Management software
Genedata Bioprocess increases the efficiency of the end-to-end pDNA development workflow, including pDNA component design and automated gene assembly, all plasmid production steps including transformationcolony picking, purification, and QC. Genedata Bioprocess tracks the history of each plasmid batch and all plate operations. It facilitates workflow automation, barcoding, and high-throughput processes, capturing all data in a structured format providing a robust foundation for predictive modeling and AI/ML applications.