Our Alexa Fluor® secondary antibodies are labeled with highly fluorescent and photostable Alexa Fluor® dyes. These antibodies are ideal for detecting specific IgG subclasses, allowing for the identification of multiple targets. To reduce background caused by cross-species reactivity, Alexa Fluor® pre-adsorbed secondary antibodies are recommended. Each product undergoes rigorous in-house testing to ensure exceptional performance in applications such as cell imaging, flow cytometry, and fluorescent western blotting. Our range includes secondary antibodies conjugated with Alexa Fluor® 405, 488, 555, 568, 594, 647, 680, 750, and 790 dyes, each offering distinct fluorescence properties for various research needs. These antibodies are validated with multiple biological samples, ensuring consistent performance across different sample types.
FEATURES
- Superior brightness: Excellent fluorescence intensity.
- Exceptional photostability: Enables extended visualization and high-quality image capture.
- pH resilience: Maintains strong fluorescence across a broad pH range.
- Enhanced water solubility: Prevents antibody precipitation and aggregation.
- Optimized quality: Calibrated dye-to-antibody ratio reduces photobleaching.
- Low background staining: Rigorous column separation process for cleaner staining.
APPLICATIONS
- Flow cytometry (Flow Cyt): Facilitates the detection and analysis of cell surface and intracellular proteins in suspension.
- Immunocytochemistry/immunofluorescence (ICC/IF): Ideal for visualizing protein localization in cultured cells using fluorescence microscopy.
- Fluorescence western blotting: Provides high sensitivity for detecting target proteins with minimal background.
- Live-cell imaging: Suitable for tracking proteins and other targets in living cells without compromising cell viability.
- Validated for use with various sample types: Ensures consistent performance across different biological samples.
FAQs
What is Alexa Fluor® used for?
Alexa Fluor® dyes are used for fluorescence labeling in various biological applications, including flow cytometry, immunohistochemistry, and microscopy, for high-sensitivity detection of specific proteins and biomolecules.
What are the common applications of Alexa Fluor® secondary antibodies?
Alexa Fluor® secondary antibodies are widely used in immunofluorescence techniques such as immunocytochemistry (ICC), flow cytometry, and ELISA, providing high brightness and minimal background fluorescence.
Can Alexa Fluor® secondary antibodies be used for live-cell imaging?
Yes, the excellent photostability and minimal background make Alexa Fluor® secondary antibodies suitable for tracking proteins and other targets in living cells.
Are Alexa Fluor® secondary antibodies suitable for super-resolution microscopy?
Yes, the high brightness and photostability of Alexa Fluor® secondary antibodies allow for precise imaging at high resolution, which is ideal for techniques like STORM and PALM.
Can Alexa Fluor® secondary antibodies be used in western blotting?
Yes, Alexa Fluor® secondary antibodies provide high sensitivity for detecting target proteins with minimal background, making them excellent for fluorescence-based western blot detection.
Can Alexa Fluor® secondary antibodies be used in combination with other fluorescent dyes?
Yes, Alexa Fluor® secondary antibodies can be combined with other fluorescent dyes for multiplexing techniques, ensuring minimal spectral overlap.