Antibody Characterization for Cell Line Development
Measuring product and critical quality attributes is an important analytical strategy for the characterization of monoclonal antibodies (mAbs). Having a robust antibody quality control process in place requires understanding and selecting the proper attributes to measure.
Antibody isotyping, concentration, structure, charge heterogeneity, post-translational modifications (PTMs), activity and stability are common targets for analysis. This requires implementing robust solutions across multiple analytical and sample preparation technologies, such as mass spectrometry, capillary electrophoresis, high-performance liquid chromatography (HPLC), automated liquid handling and data management.
The Life Sciences companies of Danaher offer all these and more. Below are a few innovative technologies that ensure a robust and reproducible antibody characterization and antibody quality control process.
- SCIEX Biophase 8800 system expedites mAb purity analysis and can be used to study consistency over time.
- SCIEX ExionLC AE system and SCIEX ZenoTOF 7600 system provide an integrated and intuitive platform for mass analysis .
- SCIEX Intabio ZT system provides multidimensional analysis, such as intact protein analysis, charge variant analysis and PTM characterization.
- Phenomenex Biozen Biologics LC Columns are used for analytical separations and analyzing product attributes.
- Automate your sample prep with a Beckman Coulter Life Sciences Biomek i-Series Automated Workstation.
- Capture and manage your data with the IDBS Polar BioPharma Lifecycle Management platform.
- Streamline mass spectrometry data analysis with Genedata Expressionist Software.