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Intabio ZT System

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Acquire Charge Variant Separation Profiles In Minutes

Achieve Highly Confident Critical Quality Attribute Identification

Identify Proteoforms That Could Impact Product Quality

The Intabio ZT system couples icIEF separation and UV detection with high-resolution mass spectrometry for peak identification.

Cut out risk, cut out information gaps. Gain multiple pieces of critical data up front.

Accelerate candidate selection by achieving separation, quantitation, and direct identification of biopharmaceutical charge variants and their proteoforms with icIEF-UV/MS via the Intabio ZT system.

Access multidimensional data in minutes instead of weeks

The Intabio ZT system couples icIEF separation and UV detection with high resolution mass spectrometry on the ZenoTOF 7600 system. Experience one integrated workflow with a single, accessible dataset containing the required information to make the right decisions, fast.

Comprehensive proteoform identification in minutes

The process of identifying individual charge variant components and interpreting their structural differences conventionally takes weeks and requires the use of multiple instruments, with multiple manual steps.

With the Intabio ZT system - separation, quantitation and identification of individual charge variants can be achieved in minutes on a single platform

Leverage key analytical functions with microfluidic chip-based technology

The Intabio ZT cartridge enables key analytical functions using proprietary microfluidic chip-based integrated icIEF-UV/MS technology.

  • Separation of charge variants with imaged capillary isoelectric focusing (icIEF)
  • Quantitation with real-time UV detection
  • Electrospray ionization for further mass spectrometry identification

Eliminate the guess work with the ZenoTOF 7600 system

Enable a more detailed characterization approach by leveraging the enhanced capabilities of the powerful ZenoTOF 7600 system with EAD fragmentation technology.

Guide decisions with multidimensional analysis using the Intabio ZT system

Let Biologics Explorer software do the heavy lifting

Maintain data continuity with a single workflow producing one, accessible dataset, where UV peaks correlate with MS peaks. Let Biologics Explorer software do the heavy lifting with pre-built workflows for charge variant analysis. Get the required information to make confident decisions, fast.

Separate, characterize, identify

High-throughput characterization with rapid multi-attribute monitoring of intact biotherapeutics and identification of charge variants throughout the drug development pipeline.

Acquire charge variant separation profiles in minutes

Acquire Charge Variant Separation Profiles In Minutes

Support high throughput analysis with 15 minute separation and enable detailed characterization on charge variant peaks of multiple monoclonal antibodies without redundant method development or fraction collection.

Achieve highly confident critical quality attribute identification

Achieve Highly Confident Critical Quality Attribute Identification

Identify common CQAs:

  • C-terminal lysine variants
  • Glycosylation
    - High mannose
    - Sialic acids
  • Deamidation
  • Glycation

Identify proteoforms that could impact product quality

Identify Proteoforms That Could Impact Product Quality

High-resolution separation and Identification of proteoforms with as little as 0.02 pI value difference.

Wherever you play in the pipeline, change the game in modern medicine

Clone selection, cell line development

Intact protein analysis, charge variant analysis, PTM characterization

Process development

Charge variants, glycoforms, PQA assessment

Formulation

Stability/degradation

Manufacturing attribute monitoring

CQA and PQA monitoring 

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Direct and rapid multi-attribute monitoring of multiple intact monoclonal antibodies with a wide isoelectric-point range of 7.3 to 9.1
This technical note demonstrates a novel integrated workflow using the Intabio ZT system. This innovative system offers direct chip-based integration of icIEF with mass spectrometry (MS), which affords confident characterization of different proteoforms of biotherapeutics and reliable quantitation of charge variants. Further, the streamlined icIEF-UV/MS platform significantly reduces the time to results from weeks to approximately 1 hour for routine samples, compared to ion exchange chromatography (IEX) with fraction collection.
pdf
Direct and rapid multi-attribute monitoring of intact monoclonal antibodies by icIEF-UV/MS
This technical note demonstrates a novel integrated workflow using the Intabio ZT system. This innovative system offers direct chip-based integration of imaged capillary isoelectric focusing (icIEF) with mass spectrometry (MS), which affords confident characterization of proteins and reliable quantitation of charge variants by icIEF-UV/MS. Further, the streamlined icIEF-UV/MS platform significantly reduces the time to results from weeks or longer to approximately 1 hour for routine samples, compared to ion exchange chromatography (IEX) with fraction collection.
pdf
Reproducibility study of an imaged capillary isoelectric focusing (icIEF)-UV/MS analysis using intact monoclonal antibody
This technical note highlights the reproducibility of a streamlined icIEF-UV/MS workflow for charge variant separation, UV quantitation, and peak identification of monoclonal antibodies (mAbs) by Intabio ZT system coupled to ZenoTOF 7600 system. The icIEF-UV/MS measurements of NISTmAb were performed across 45 injections with 3 cartridges using the Intabio ZT system, resulting in high inter- and intra-cartridge reproducibility (%CVs <5%).
pdf
A comprehensive workflow to characterize deglycosylated NISTmAb using imaged capillary isoelectric focusing (icIEF)-UV/MS
This technical note presents a comprehensive analysis of deglycosylated NISTmAb (dgNISTmAb) using a novel integrated icIEF-UV/MS workflow. This innovative Intabio ZT system offers direct chip-based integration of icIEF with mass spectrometry (MS), which affords the confident characterization of proteins and reliable quantitation of charge variants. The streamlined icIEF-UV/MS platform significantly reduces the time to obtain results from weeks or longer to approximately 1 hour for routine samples, compared to ion exchange chromatography (IEX) with fraction collection