Introducing our easy-to-use comprehensive SpectraMax® line of microplate readers. The flexibility of these plate readers allows you to configure them according to your specific requirements, adapting them to various experimental setups. From fundamental tasks like ELISA assays to nucleic acid and protein quantitation to more intricate endeavors such as gene expression studies and enzyme kinetic assays, our automated microplate readers cater to a wide spectrum of applications. Elevate your research with our seamlessly integrated microplate readers, designed to provide efficiency and convenience at every step.
Features
Essential Features of High-quality Microplate Readers
- Our reader stands out with exceptional microplate reader performance, ensuring accurate and reliable results for your research needs. It excels in high-throughput screening, efficiently analyzing a large number of samples. Powered by advanced detection technologies, it delivers precise measurements across various detection modes, elevating the quality of your experimental data.
- When it comes to quality, our microplate reader is unmatched. It's engineered to meet the highest standards, guaranteeing consistent and reproducible outcomes. The incorporation of cutting-edge automation streamlines your workflow, reducing human error and enhancing result reliability. Whether you're utilizing absorbance, fluorescence, luminescence, or other detection modes, our microplate reader maintains unparalleled quality throughout your experiments.
- Built to last, our microplate reader is designed for long-term durability. Its robust construction ensures it can withstand the demands of frequent use in diverse laboratory settings. This durability, combined with its advanced features, makes it a reliable workhorse for your research endeavors.
Applications
Applications of Microplate Readers
ELISA microplate readers find valuable application in ELISA assays, as these are used to accurately quantify target molecules by measuring the absorbance of enzymatic reactions in microplate wells.
- In cell line development, microplate readers are utilized to assess cell viability, proliferation, and protein expression levels.
- In cell line development, microplate readers are utilized to assess cell viability, proliferation, and protein expression levels.
- Microplate readers are indispensable tools in antibody screening, as they enable high-throughput quantification of antibody-antigen interactions.
- Microplate readers play a vital role in plasmid DNA (pDNA) DNA template testing during mRNA characterization, enabling efficient quantification of template concentrations through absorbance measurements and ensuring the quality and accuracy of mRNA synthesis processes.
- Microplate readers are essential tools for bioassay screening, facilitating the high-throughput analysis of biological responses by measuring various readouts such as fluorescence, luminescence, or absorbance.
- Microplate readers are indispensable tools in drug discovery and development, facilitating the high-throughput screening of compounds and their interactions with biological targets, and enabling precise oligonucleotide characterization for therapeutic development.
FAQs
What factors should I consider when choosing a plate reader for my life science research?
Consider detection modes, throughput needs, reader accessories, sensitivity, plate formats, software capabilities, and temperature control to ensure the selected plate reader suits your assay requirements, budget, and automation needs while maintaining high-quality data output.
How does a microplate reader work?
A microplate reader uses a range of wavelengths to detect biological, chemical or physical signals emitted by the samples contained within a microplate. These signals are then quantified and analyzed, providing insights into various biological and biochemical processes within the samples, such as protein interactions, enzymatic reactions, or cell viability assessments.
How are microplate readers used in Polymerase Chain Reaction (PCR) workflows?
PCR is a method used to quickly and accurately amplify copies of DNA segments. In gene expression experiments, a technique called qPCR, allows for monitoring of real-time fluorescence signals during amplification. Microplate readers are used post PCR to assess quality and quantity of amplified material, perform high-throughput genotyping, analyze melting curves, and quantify reporter gene expression. Microplate readers streamline PCR-based experiments and enhance data collection accuracy in molecular biology research and diagnostics.
How is a spectrophotometer different from a Biotech microplate reader?
A microplate reader is optimized for high-throughput analysis of samples in microplate formats, offering capabilities for fluorescence, luminescence, and time-resolved fluorescence measurements, ideal for assays like ELISA and gene expression studies. In contrast, a spectrophotometer is versatile for absorbance measurements in cuvettes, accommodating single-sample measurements. It is commonly used for applications like nucleic acid quantification and protein analysis.
What is an ELISA microplate reader used for?
An ELISA plate reader measures the absorbance or fluorescence signals of enzyme reactions, providing quantifiable data that helps detect and quantify the presence of specific antigens or antibodies in a sample.
Differentiate between the different types of dedicated microplate readers.
Absorbance Microplate Readers: We carry absorbance spectrophotometers and multi-well plate readers for UV-Vis absorbance detection. These 96-well microplate readers or 384-well plate readers offer higher throughput, measuring absorbance in microplates by vertically passing light through each well.
Fluorescence Microplate Readers: Multi-wavelength measurement is achieved without filters through our top- and bottom-reading fluorescence microplate readers. These readers feature dual monochromators, offering flexibility in establishing the best excitation and emission parameters for fluorescence intensity assays. Cell-based assay sensitivity is enhanced through well scanning at multiple points.
Luminescence Microplate Readers: Our specialized luminescence microplate plate reader, known as a luminometer, is designed for single-mode luminescence detection. Luminescence detection is known for its heightened sensitivity compared to absorbance or fluorescence detection methods. These luminescence plate readers are exceptionally well-suited for quantifying various assays.
Microplate washers: Microplate washers simplify usage with walk-up convenience and user-changeable heads. They minimize processing time, reduce residual volume, and simplify operation while minimizing wash duration.
Multi-mode readers: While a single-mode plate reader is a suitable initial choice, opting for an upgradable multi-mode reader that encompasses absorbance, fluorescence, and luminescence functions can effectively cater to your evolving laboratory requirements. Our multi-mode plate readers offer customizable detection for fluorescence polarization (FP) and time-resolved fluorescence (TRF) , and options for HTRF and AlphaScreen. Additionally, these readers provide the opportunity to integrate upgradeable modules such as western blot capabilities, cell imaging, and fast kinetics with injectors.