Our microplate luminometers are cutting-edge single-mode luminescence microplate readers for precise luminescence detection. When a luminescence reaction is established within a microplate, these luminometers are employed to gauge the generated light quantity.
Overview
Enhanced sensitivity and Accuracy in Luminescence Detection
These luminometers stand out as advanced devices renowned for their heightened sensitivity compared to absorbance or fluorescence detection methods. While luminescence assays characterized by long-lasting signals can be captured by any luminescence reader, flash assays featuring short-lived signals necessitate a reader equipped with injectors. The microplate is positioned within a light-secure reading chamber, and individual photomultiplier tubes (PMTs) successively capture light emissions from each well. The luminescence measurements are quantified as relative light units (RLU).
The advanced filter design enhances detection accuracy and extends the detection limit, making it an indispensable tool for high-quality luminescence measurements in various research applications. The built-in software of the luminescence plate reader enhances its utility across a spectrum of applications, from assessing cell viability to facilitating hygiene monitoring programs and quality supervision. By offering a seamless interface for data analysis and interpretation, this software empowers researchers to efficiently derive insights from their luminescence measurements, making the luminometer, plate reader, an indispensable tool in diverse scientific and analytical endeavors. Initiating with a single-mode plate reader is a favorable beginning. Opting for an upgradable multi-mode reader that encompasses absorbance, fluorescence, and luminescence capabilities can effectively cater to your forthcoming laboratory requirements. Designed as versatile luminometers, these instruments excel in assessing a diverse array of assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester assays.
Features
Essential features of our Exclusive Life Science Research Luminometer
- The luciferase plate reader excels in its smooth operation, offering user-friendly interfaces that facilitate seamless assay execution. The integration of advanced technologies ensures that tests can be conducted swiftly and efficiently.
- The handheld device boasts an impressive dynamic range, capturing a broad spectrum of luminescence signals. From faint glows to intense flashes, the reader's sensitivity accommodates various assay requirements, enabling precise data collection across diverse sample types.
- Rigorously tested for reliability, our microplate readers for luminescence exhibit consistent and accurate performance. Their quality assurance measures not only guarantee precise measurements but also establish these devices as durable assets in the laboratory, with the ability to deliver dependable results over an extended period.
Applications
High-throughput applications of our Luminescence microplate Readers
- Luminescent ATP assays offer a swift and sensitive method to assess cell viability and cytotoxicity, swiftly quantifying viable cell counts and gauging the impact of experimental interventions. The use of ATP luminometers enables high-throughput execution of these cell-based assays.
- The QuantiGlo Chemiluminescent VEGF Immunoassay employs a solid phase ELISA technique to quantify VEGF165 levels via a chemiluminescence reaction, with readings acquired using our chemiluminescence plate reader, suitable for both flash and glow applications in 384- and 96-well plate formats.
- Mycoplasma contamination is effectively and sensitively detected using Mycoplasma Detection Assays on our microplate readers, offering swift outcomes through a straightforward add-and-read approach and uncomplicated result interpretation.
- Our luminometers enable accurate quantification of cellular ROS levels using a luminescence-based assay, shedding light on oxidative stress and its impact on cellular biology.
FAQs
Define luminescence.
Luminescence entails the release of light from a material due to either a chemical reaction (chemiluminescence) or an enzymatic reaction (bioluminescence).
What are the key characteristics of our featured SpectraMax® L luminescence detection microplate reader?
Our SpectraMax® L Luminescence Microplate Reader is a highly sensitive luminometer featuring programmable injector options for 96- and 384-well microplates. Its unmatched sensitivity facilitates the detection of even the faintest samples, while its extensive nine-order dynamic range prevents saturation issues. The integration of injectors ensures robust performance, complemented by the availability of validation and compliance tools. The SpectraMax® L Microplate Reader finds utility in diverse areas, including drug discovery & development, cellular signaling, evaluating the hygienic state of surfaces, etc.
What factors should I take into account when purchasing a luminometer? Why should one choose our luminescence plate reader?
When buying a luminometer, it's important to consider
- Sensitivity
- Dynamic range
- Compatibility with microplate formats
- Detection modes
- Software capabilities
- Usability
Luminometers are versatile tools with applications ranging from hygiene monitoring to advanced biological research.
Our luminometer stands out with
- real-time apoptosis and necrosis assessment capabilities.
- dual luciferase reporter (DLR) assay certification.
- applications ranging from hygiene monitoring to advanced biological research.
- Detection of emitted light, including the faintest flashes of light, amidst various environmental conditions, effectively distinguishing luminescent signals from ambient and visible light.
These features empower users to conduct comprehensive studies and gain insights into various biological processes.
Contact our team for help in choosing the luminometer for your application.
What is the function of our luminometers?
Our luminescence plate reader, also known as a luminometer, measures the emitted light resulting from the release of photons accurately during a range of biochemical reactions. This functionality enables researchers to quantitatively assess and analyze processes involving bioluminescent and chemiluminescent processes, offering valuable insights into various biological phenomena.
How do our luminometers contribute to Cytotoxicity assays and Drug Discovery & Development?
Our luminometers play a crucial role in advancing Cytotoxicity assays and Drug Discovery & Development. Specifically, they facilitate luminescent ATP assays, providing a rapid and sensitive method to assess cell viability and cytotoxicity. This allows for swift quantification of viable cell counts and the evaluation of the impact of experimental interventions. Moreover, our chemiluminescence plate reader, designed for both flash and glow applications, contributes significantly to drug discovery efforts. It employs solid-phase ELISA techniques to quantify specific biomarkers, such as VEGF165, aiding in the screening and analysis of potential drug candidates.
How is NanoBRET/BRET technology utilized with our luminescence Microplate Reader?
NanoBRET technology is effectively employed with our SpectraMax i3x Multi-Mode Microplate Reader to measure protein-protein interactions, exemplified by its application in studying the p53-MDM2 interaction. This technique, utilizing a bioluminescent donor and a fluorescent acceptor, enables precise detection and analysis of NanoBRET signals, providing valuable insights into molecular interactions crucial for drug discovery research.
How are Single/Dual-Luciferase Reporter Gene Assays used with our luminometers?
Single/Dual-Luciferase Reporter Gene Assays are employed with our luminometers to investigate gene expression linked to cellular pathway activation. By transfecting cells with a plasmid containing both the reporter gene and a region of interest, such as a promoter, these assays enable the measurement of expressed reporter gene levels, offering insights into the activity of specific cellular pathways. Our microplate readers, including the SpectraMax iD5, FlexStation 3, and SpectraMax L, support various formats for dual-luciferase assays in both 96- and 384-well plates, allowing for the monitoring of processes such as NF-kB activation. The SpectraMax Glo Steady-Luc Reporter Assay Kit is utilized to measure luciferase expression, providing a comprehensive solution for researchers studying gene regulation and transcriptional control elements.
What types of assays can be performed with our luminescence Microplate Reader?
Our luminescence microplate readers support a wide array of assays, including but not limited to
- ATP-based cell viability assays
- Chemiluminescent ELISA methods
- Cytotoxicity assays
- Mycoplasma monitoring
- NanoBRET/BRET
- Reactive Oxygen Species (ROS) assays
- Single/Dual-Luciferase Reporter Gene Assays
- Dual luciferase reporter gene assays
- G protein-coupled receptor (GPCR) assays via aequorin
- Bioluminescence resonance energy transfer (BRET)
- Acridinium ester assays.
This comprehensive range of capabilities allows researchers to explore various biology.
What types of samples can be analyzed with our luminescence microplate readers?
The luminescence microplate readers excel in analyzing a broad spectrum of samples, accommodating everything from faint glows to intense flashes with their unparalleled sensitivity. This capability allows for precise data collection across diverse sample types, ensuring the detection of even the faintest signals while preventing saturation issues through an extensive nine-order dynamic range.
Are there specific requirements for sample preparation when using luminescence plate readers?
Sample preparation for luminescence plate readers varies depending on the specific assay being conducted. Generally, it involves the proper handling and loading of samples onto the microplate, ensuring appropriate reagent addition, and following the assay protocol provided for the chosen luminescence application. Specific requirements may include adjusting cell density, incubation times or adding specific substrates for the detection of luminescent signals. Careful adherence to the assay's recommended procedures is crucial to obtain accurate and reliable results.
Get in touch with our life sciences experts to learn more about the particular sample preparation requirements tailored to your research demands.
Can the luminometers be used for high-throughput screening?
Yes, our luminometers are well-suited for high-throughput screening applications. Specifically, luminescent ATP assays, which provide a rapid and sensitive method for assessing cell viability and cytotoxicity, allow for the swift quantification of viable cell counts and the evaluation of experimental interventions. The use of ATP luminometers facilitates the high-throughput execution of these cell-based assays, making them valuable tools for screening large numbers of samples in a time-efficient manner.
Can the luminometers be integrated with other laboratory equipment or software systems?
Renowned for heightened sensitivity compared to absorbance or fluorescence methods, these advanced luminometers can be seamlessly integrated with other laboratory equipment or software systems. With luminescence assays capturing both long-lasting and short-lived signals and enhanced by advanced filter design, the built-in software empowers efficient data analysis and interpretation, making these luminometers indispensable tools across various research applications.
What technical support, training or resources are available to help you learn how to use the SpectraMax L luminescence Microplate Reader?
We provide comprehensive technical support, training, and resources to facilitate your mastery of the SpectraMax L luminescence Microplate Reader. Leveraging over 40 years of plate reader expertise and life science research, our Resource Hub offers a wealth of application-focused content, including luminescence application notes. Additionally, we offer a complete solution of validation tools and services, along with a diverse range of consumables and assays, ensuring that all your luminometer lab needs are met. Our highly qualified teams are dedicated to assisting you, offering remote or on-site product demonstrations, webinars, and more to help you overcome your research challenges effectively.
Contact our team to know more.
What is the detection range and sensitivity of the luminescence plate readers?
Our microplate luminometers, exemplified by the SpectraMax L, offer cutting-edge single-mode luminescence detection with a detection range and sensitivity that set them apart. Renowned for their heightened sensitivity compared to absorbance or fluorescence detection methods, these advanced devices are capable of capturing luminescence signals with remarkable precision. Luminescence assays characterized by long-lasting signals can be efficiently detected by any luminescence reader, while flash assays featuring short-lived signals require a reader equipped with injectors.
The microplate is positioned within a light-secure reading chamber, and individual photomultiplier tubes (PMTs) successively capture light emissions from each well. Luminescence measurements are quantified as relative light units (RLU), and the unmatched sensitivity of our luminometers facilitates the detection of even the faintest samples. The extensive nine-order dynamic range prevents saturation issues, ensuring accurate and reliable results.
Moreover, the advanced filter design enhances detection accuracy and extends the detection limit, making our luminometers indispensable tools for achieving high-quality luminescence measurements in various research applications.
What are the advantages of using luminometers compared to other detection methods like absorbance or fluorescence?
- Luminometers offer heightened sensitivity, low background noise, and a wide dynamic range compared to absorbance or fluorescence methods, making them ideal for various assays with diverse analyte concentrations.
- Their rapid response, prolonged signal duration, and independence from external light sources contribute to their versatility and suitability for high-throughput screening applications in research and diagnostics.