The Gemini XPS and EM Microplate Readers are at the forefront of fluorescence detection, utilizing dual monochromators and multi-point well scanning for high-performance fluorescence intensity assays. The innovative design of this analytical instrument eliminates the need for filters, offering a wide range of excitation and emission wavelength options, streamlining maintenance and maximizing performance.
Overview
Our fluorescence plate readers represent cutting-edge solutions for fluorescence detection and multi-wavelength measurements, establishing them as an essential instrument in fluorescence spectroscopy labs. With a unique dual monochromator setup, our fluorescence readers provide unmatched flexibility in determining the most suitable excitation and emission settings for precise fluorescence intensity assays. This innovation enables researchers to conduct multi-point well scanning, significantly enhancing the sensitivity of cell-based assays by capturing fluorescence measurements with photons from multiple points within each microplate well. What sets our fluorescence microplate readers apart is their ability to perform a unique calibration against an internal standard, allowing for the correct comparison of relative fluorescence units (RFUs) across different samples. Furthermore, the fluorescence microplate readers excel in maintaining consistent temperature regulation, which is crucial for monitoring temperature-sensitive reactions, and they eliminate the need for filters by offering a broad range of excitation and emission wavelength selections from 250 nm to 850 nm.
Features
Essential features of our Fluorescent Microplate Reader (Spectrofluorometer)
- The microplate fluorometers stand out for their exceptional performance, driven by dual monochromators and multi-point well scanning. They enable precise fluorescence intensity assays and enhanced sensitivity in cell-based experiments while also offering advanced capabilities for fluorescence polarization measurements.
- Our fluorescence microplate readers, which accommodate various plate formats, boast a crucial attribute known as dynamic range. This attribute defines the reader's ability to accurately and precisely capture, and quantify an expansive range of signal intensities, spanning from the faintest detectable signals to levels of saturation, all while upholding measurement accuracy and avoiding signal distortion.
- Microplates for fluorescence-based assays exhibit remarkable durability through consistent temperature regulation, ranging from ambient to 45°C. This reliability supports monitoring temperature-sensitive reactions over extended periods, assuring researchers of their longevity. Furthermore, these spectrofluorometers boast exceptional endurance, ensuring efficacy throughout their lifetime, and are adept for time-resolved fluorescence experiments that require precise temperature control.
Applications
Fluorescent Microplate Readers and Spectrophotometers Applications
- These fluorescence detectors are employed in cell-based assays to study cellular processes, such as signaling pathways, gene expression, and protein interactions. They can measure fluorescence changes in live cells or fixed samples.
- Fluorescence-based assay types are employed to measure enzymatic activity. Substrates are modified with fluorophores, and changes in fluorescence intensity are indicative of enzyme activity levels.
- These products are used to study biomolecular interactions like protein-protein interactions, protein-DNA binding, and ligand-receptor interactions. Fluorescent labels are attached to molecules of interest, and the reader detects changes in fluorescence upon binding.
- Fluorescence assay technologies are used to assess cell viability and cytotoxicity by measuring the fluorescence emitted by dyes that indicate cell health or membrane integrity.
- The technique helps in analyzing the composition and chemical changes on the surface of materials exposed to corrosive environments, assisting in corrosion mitigation strategies.
- Our fluorescence settings play a pivotal role in drug discovery by utilizing an exciting beam of light to activate fluorescently labeled molecules in assays.
- Fluorescence readers equipped with precise calibration procedures, offer accurate quantitation of nucleic acids. By creating calibration curves based on known absorption coefficients and absorption spectra, potential calibration errors are minimized, ensuring reliable and consistent quantification of DNA and RNA samples.
- Fluorometers and spectrophotometers excel in protein analysis through their exceptional sensitivity and ability to measure protein concentrations within a wide spectral range. These instruments provide accurate quantitation with analog output by utilizing pulse or continuous light sources and quantum efficiency calculations. They enable researchers to detect and quantify proteins at single wavelengths, ensuring high analytical efficiency and reliable transmission of protein-related data for comprehensive analysis.
FAQs
What is fluorescence spectroscopy used for?
Our fluorescencespectrometers serve a wide range of applications, including analyzing molecular structures, protein behaviors, cellular processes, and environmental factors while also aiding medical diagnostics, drug discovery, and material characterization.
How can I receive assistance in choosing the most suitable fluorescence reader for my research requirements?
Our team of experts is available to provide guidance based on factors such as your research objectives, preferred assay types, sensitivity needs, and budget constraints. Please contact our support team, and we'll help you identify the ideal commercial instrument solution tailored to your research endeavors.
What are the key aspects of fluorescence analysis covered by our instrumentation?
Our instrumentation encompasses a wide range of fluorescence analysis techniques, including fluorescence lifetime measurements for studying molecular dynamics, investigating the transition behaviors of polyatomic fluorescent molecules, characterizing the fluorescent properties of various compounds, performing emission scans to gather comprehensive data, and generating detailed emission spectra. Additionally, our instruments address the phenomenon of reabsorption of fluorescence, ensuring a thorough understanding of fluorescence emission processes across various samples and scenarios.
What range of excitation wavelengths does our instrumentation cover?
Our instrumentation covers a wide excitation wavelength range to accommodate diverse research needs. Please refer to our specifications or contact our support team for detailed information regarding the excitation wavelength range of our equipment.
How do Fluorescence Microplate Readers work?
Fluorescence microplate readers operate by measuring the fluorescent light emitted from samples labeled with fluorescent dyes or molecules. In the process, a specific wavelength of light is directed onto the sample, exciting the fluorescent molecules. These molecules then emit light at a longer wavelength, which is detected by the microplate reader's photodetector. The intensity of the emitted fluorescence correlates with the concentration of the target substance in the sample. This enables quantitative analysis of various biological and chemical processes, making fluorescence microplate readers valuable tools in fields such as molecular biology, drug discovery, and clinical diagnostics.
What types of samples can be analyzed using fluorescence microplate readers?
Our fluorescence microplate readers are versatile instruments capable of analyzing a wide range of samples across various scientific disciplines. Common sample types include:
Cellular Assays - Analysis of cellular processes, such as gene expression, apoptosis, and cell viability.
Enzyme Activity Assays - Monitoring enzymatic reactions in drug discovery or biochemistry research.
Protein Quantification - Determining protein concentrations using fluorescently labeled proteins or dyes.
DNA/RNA Quantification - Quantifying nucleic acids in molecular biology applications.
Immunological Assays - Detecting and quantifying specific antibodies or antigens in immunological studies.
These fluorescence microplate readers offer flexibility, enabling researchers to perform a diverse array of assays in high-throughput formats.
How can we improve Fluorescence detection?
Fluorescence detection can be improved by:
Optimizing experimental conditions such as excitation and emission wavelengths for the specific fluorophores used.
Employing advanced signal processing techniques.
Reducing background noise.
Choosing sensitive detectors.
Using high-quality optics.
Minimizing sample autofluorescence.
Employing sophisticated data analysis algorithms to improve measurement accuracy and sensitivity.
What is the range of Fluorescence detection?
The fluorescence detection range of organic dyes typically spans from low picomolar to high micromolar concentrations (10-8–10-11) of fluorophores. The sensitivity depends on factors such as the specific fluorophore used, the instrumentation, and the sample matrix. Advanced fluorescence detection systems can detect even lower concentrations, allowing for highly sensitive analyses in various scientific applications, including molecular biology, drug discovery, and clinical diagnostics. Detection limits can vary, but modern instruments often achieve high sensitivity, enabling the quantification of trace amounts of fluorescent molecules in complex samples.
What are the different types of labels used in Fluorescence detection?
Typically, the labels used for fluorescence detection are:
- FITC or fluorescein isothiocyanate (FITC)
- Dyes like Rhodamine B or Lucifer Yellow
- Labels developed from lanthanide chelates (for time-resolved fluorescence)
What are the commonly used fluorescent probes?
Commonly used fluorescent probes in fluorescence microplate readers include:
Fluorescein Derivatives: Fluorescein-based probes are widely employed in various assays as they offer a broad range of excitation and emission wavelengths.
Rhodamine Derivatives: Known for their bright and stable fluorescence, rhodamine-based probes are versatile and suitable for different applications.
Quantum Dots: Nanoscale semiconductor particles with tunable fluorescence properties, providing high brightness and multiplexing capabilities.
Fluorescent Nucleic Acid Stains: Examples include SYBR Green and DAPI (used for quantifying and visualizing DNA and RNA in molecular biology assays).
Fluorescent Antibodies: Antibodies labeled with fluorescent dyes for immunofluorescence assays, enabling specific target detection.
These probes cover a range of applications, allowing for sensitive and multiplexed fluorescence measurements in microplate-based assays.