These photomultiplier tube-based spectrophotometers and plate readers feature eight-channel optics, enabling rapid plate reads in as fast as five seconds. You have the option to use either a microplate or a standard cuvette for your experiments, with the flexibility to choose between 96-well or 384-well microplates. The inclusion of PathCheck Sensor technology allows for accurate measurement of the optical path length of samples. Enhancing the capabilities, the SoftMax Pro Software serves as an industry-leading tool for data acquisition and analysis. The system also ensures GxP compliance and validation, with measurements verified through the use of the SpectraTest validation plate and associated data analysis software. Additionally, the system can be integrated with stackers and other automation systems for increased efficiency and throughput. Absorbance (A) or optical density (OD) signifies the light absorbed by a solution, while transmittance represents the light passing through. These are key in spectrophotometry. Molecular Devices pioneered UV-capable microplate readers, enabling UV measurements in microplates. This innovation greatly popularized microplate-based measurement of DNA, RNA, and proteins.
Features
Essential features of UV-Vis Microplate Spectrophotometer
- Our spectrometer and microplate reader are designed to boast a wide dynamic range and quantitative analysis prowess, including 96-well microplate reading for high-throughput absorbance detection across various concentrations, and possess features to help you save time.
- Renowned for their superior quality, the microplate reader and spectrophotometer utilize monochromator-based wavelength selection and robust optics to ensure precise measurements and minimized interference, accompanied by built-in quality control features for consistent performance.
- Engineered with sturdy construction and designed for long-term stability, lab spectrophotometers offer reliability throughout extended lab use, backed by comprehensive warranties and dedicated customer support.
Applications
Spectrophotometer and Microplate Reader Applications
- Standard spectrophotometers are crucial for quantifying molecular densities in solutions by measuring the extent of light absorption, aiding in molecular characterization.
- Absorbance microplate readers play a pivotal role in drug discovery, enabling researchers to assess the interactions between compounds and target molecules through absorbance measurements.
- These UV-Vis microplate readers are extensively used for protein quantification assays, determining protein concentrations in samples based on their absorbance characteristics.
- In kinetic assays, the microtiter plate reader and spectrophotometer track the time-dependent changes in absorbance, facilitating the study of enzymatic reactions, substrate conversions, and other dynamic processes.
- For cell-based assays, UV plate readers are employed to analyze cell viability, proliferation, and metabolic activity by measuring absorbance changes in response to specific treatments.
- Spectrophotometric plate readers are central to colorimetric assays, where chemical reactions generate colored products that can be quantified through absorbance measurements.
FAQs
What sets our 96-well spectrophotometer apart in life science research?
Our 96-well microplate reader stands out for its advanced technology and precision. With the ability to simultaneously analyze 96 samples in individual wells of a microplate, it offers high-throughput capabilities for various assays. The reader can measure with accuracy; this feature and its user-friendly interface make it a valuable tool for applications ranging from DNA/RNA analysis to protein quantification. Backed by our reputation for quality, this spectrophotometer is a dependable choice for laboratories seeking efficient and reliable sample analysis.
What are the advantages of our optical density plate readers?
The advantages of our microplate readers include cutting-edge technology, exceptional accuracy, reliable results, user-friendly interfaces, versatile application support, and the backing of our strong reputation for quality and innovation.
How is a spectrophotometer used to measure absorbance data?
A spectrophotometer measures absorbance data by passing a specific wavelength of light through a sample solution and detecting the intensity of transmitted light. The resulting absorbance value is used to determine the concentration of absorbing molecules in the solution, aiding in various scientific and analytical applications.
How is a spectrophotometer different from a microplate reader?
A regular spectrophotometer gauges the absorbance of individual samples sequentially. It usually involves putting the sample in a cuvette, through which light travels horizontally. On the other hand, an absorbance plate reader provides increased efficiency, as it can assess the absorbance of numerous samples within microplates, often containing 96 or 384 wells. This is achieved by directing light vertically through each well.
Differentiations among our spectrophotometric multiwell plate readers.
SpectraMax QuickDrop Micro-Volume Spectrophotometer: The microplate reader and spectrophotometer quantify minute quantities of DNA, RNA, oligos, and proteins. The DNA spectrophotometer's compact size and user-friendly touchscreen enable effortless laboratory configuration, requiring minimal cost and effort investment.
SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers: This automated spectrophotometer employs Molecular Devices' PathCheck Sensor technology, which is unaffected by temperature changes, to measure sample depth in a microplate. The microplate ELISA photometer excels with round-bottom, flat-bottom, or half-area well plates, providing accurate dual-wavelength readings for simplified data analysis.
EMax Plus Microplate Reader: The microplate photometer offers a sturdy entry-level solution featuring eight filters for visible-wavelength absorption measurements like protein quantification, cell viability, and ELISA. It accommodates flat and round 96-well microplates and maintains accuracy through automatic lamp calibration before each reading.