Stable Transfection for Cell Line Development
Transfection is the process of delivering cargo, such as a plasmid or other expression vectors, via a non-viral mechanism across the cell membrane to induce gene expression. Transfection is not to be confused with transformation, which is the uptake of environmental plasmid DNA by bacteria, or transduction, which leverages viral vectors.
There are two classifications of transfection: stable and transient. Transient transfection is short-term, while stable transfection ensures that gene expression is preserved in daughter cells after cell division.
During the cell line development process, stable transfection is leveraged to integrate the gene of interest via plasmids or other expression vectors into the host cell genome for monoclonal antibody expression. The Beckman Coulter Life Sciences Biomek i-Series Automated Liquid Handler can automate transfection workflows, including transfection reagent pipetting and handling and cell growth. It can also prepare assay samples to quantify transfection efficiency and post-transfection cell death. To obtain cell count and viability data, integrate a Beckman Coulter Life Sciences Vi-CELL BLU Cell Viability Analyzer during cell recovery. Stable cell lines can be selected using a Molecular Devices ClonePix 2 Mammalian Colony Picker.
Digital workflows for structured data capture can increase efficiency and save significant time. The IDBS E-WorkBook and Polar BioPharma Lifecycle Management platform can support data capture and integration for streamlined reporting and data sharing with internal or external teams.
Stable Transfection Products
ClonePix® 2 Mammalian Colony Picker
A transformative cell line development workflow with automated clone screening and optional monoclonality assurance on day 0*
View ProductsVi-CELL BLU Cell Viability Analyzer
Fully automated trypan blue dye exclusion method High throughput 24 position carousel Supports 96 well plate Faster analysis time compared to Vi-CELL XR Single sign-on with Active Directory Facilitates 21 CFR Part 11
View ProductsBiomek i5 Automated Workstation Liquid Handler
<p>Optimize dependability and walk-away time. The Biomek i5 is a recent addition to our liquid handling portfolio, representing the best of what has made Biomek an industry-leading brand - combined with enhancements suggested by customers around the globe.</p> <ul> <li>Ideal for medium- to high-throughput workflows</li> <li>25 deck positions</li> <li>0.5 µL-1,000 µL (Multichannel) or 0.5 µL-5,000 µL (Span-8) pipetting volume ranges</li> <li>Single Multichannel head (96/384) or Span-8 pipetting with gripper</li> </ul>
View ProductsBiomek i7 Automated Workstation
The most powerful and comprehensive Biomek available. High-throughput 45 deck positions 0.5 - 5,000 µL pipetting volume range Single or dual heads
View ProductsGenedata Biopharma Platform: Biopharma Automation & AI
Genedata transforms biopharma R&D through automation, advanced analytics, and AI. As the first biologics-native technology company, we lead digitalization and enable data-driven decision-making for biopharma organizations worldwide. The Genedata Biopharma Platform combines a unified data model with workflow automation and embedded AI to accelerate and optimize end-to-end discovery and development of biologics and other modalities.
View ProductsIDBS Polar
An enterprise lab informatics software platform with ELN, LES and LIMS capabilities designed to power scientific innovation.
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