STELLARIS FALCON FLIM Microscope

Modern research investigates how molecules interact to accomplish vital tasks. FLIM-FRET is the gold standard for exploring these interactions.

STELLARIS FALCON sets a new speed standard for FLIM instruments.

It enables FRET in highly dynamic cellular events. You can acquire and interpret FRET data in your daily experiments.

Biosensors are powerful reporters for metabolic activity, signalling mechanisms, pH, and microenvironment changes.

STELLARIS FALCON provides access to information contained in fluorescence lifetime, even for ultrafast events such as membrane potential dynamics. This information complements the spectral fluorescence intensity imaging and TauSense modes delivered by the STELLARIS system.

Autofluorescence can be an issue in conventional imaging. STELLARIS FALCON turns it into valuable information. You can now transform autofluorescence into a reporter for metabolic status, cell differentiation, and cancer development.

Moreover, STELLARIS FALCON enables imaging contrast in living tissue, where fluorescence labelling is often unspecific or destroys physiological conditions.

Fluorescence labelling is the standard way to differentiate intracellular structures. Spectral separation is very powerful, but sometimes limited when the emission spectra are too close.

With STELLARIS FALCON, you can exploit the full potential of fluorescence lifetime imaging to separate multiple fluorescent probes using exponential fitting, pattern fitting, and the new FLIM phasor separation analysis.

Interactive image: Cytoskeleton structures distinguished by lifetime contrast. Vimentin immunolabelled with Alexa Fluor 555 (green), and tubulin immunolabelled with Alexa Fluor 546 (blue). The fluorophores are spectrally very similar, but they are separated using fluorescence lifetime information. Image size: 512 × 512 pixels.

The STELLARIS FALCON microscope overcomes the speed limitation of FLIM and opens the door to fast lifetime data.

Up to now, functional information extracted from fluorescence lifetime data of fast processes was hard to obtain due to the technical constraints of FLIM. FLIM acquisition speed was at least ten times slower than recording the confocal intensity.

Using STELLARIS FALCON fast lifetime contrast imaging, you can follow dynamic processes in cells at the appropriate pace. These capabilities are possible thanks to a new way to measure time using TCSPC (Time-Correlated Single Photon Counting) together with intelligent algorithms for data handling and analysis.

Application note in Nature: SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM.

Combining FLIM with other modalities was never as easy as with STELLARIS FALCON. Until now, researchers had to cope with complex wiring and cumbersome file transfer tasks. With STELLARIS FALCON, you can integrate lifetime information into your standard confocal workflow.

STELLARIS FALCON is fully integrated into the LAS X acquisition and analysis software. It can record FLIM in four spectral channels simultaneously and up to ten channels sequentially. STELLARIS FALCON gives you access to lifetime contrast in 3D stacks, time-lapse sequences, and even large mosaic-tiling formats.

With LAS X NAVIGATOR, you can expand your viewing area up to 10,000 times, saving valuable time when identifying"overviewimage_7">