High-throughput colony screening and picking is a must for efficient and transformative cell line development. Our colony picker is a fully automated system that ensures you obtain high-quality clones without compromising cell viability or risk of colony disturbance. The sophisticated colony picker system enables a screening speed of > 10,000 clones per day, allowing you to screen more clones in less time with monoclonal verification on day zero. The process is faster than labor-intensive limiting dilution and FACS techniques.

You have the flexibility to screen and select mammalian clones based on variable parameters that meet your experimental requirements, such as antigen-specificity, protein productivity, expression levels, and cell viability of tagged recombinant proteins. Further, the system is fully integrated with barcode reading, plate handling, and colony picking.

All data linked to each colony, including images of picked clones or cells, are collected and saved for downstream applications.

Features

Key Features of Mammalian Colony Picker

Our automated mammalian screening systems come with a host of features to suit your range of applications:

• Efficient data collection using high-resolution white light and fluorescence imaging.
• Custom image detection algorithms, optimized for high resolution images.
• Z stack imaging for resolution of single cells on day zero.
• Imaging, picking and single cell analysis processes
• Specific pin design for suspension cells and adherent cells.
• High source and destination plate capacity (10 plates each) allows for ample walk-away time.
• Drive position encoders allow for high precision picking.
• Internal barcode enables easy tracking of colony formation from a single cell for monoclonality assurance.
• Screen 10X more clones in 2.7x less time compared to limiting dilution.
• System equipped with UV sterilization, and multi-wash baths offers sustainable, cost effective and environmentally friendly sanitization option.

Applications

Applications - Redefining clone screening and selection

Our specialized mammalian colony picker enables you to screen and select relevant clones. Here are some areas where our products are of tremendous use:

• The colony picker has an essential role in cell line development to generate biopharmaceutical molecules. It allows efficient screening of rare, high-producing cells faster than the traditional approaches.
• These mammalian colony pickers offer an automated approach to screening extensive cell populations, enhancing the likelihood of identifying rare high-affinity binders or highly productive cells while screening a transfected cell population for high-value clones with elevated cell surface expression of particular receptors.
• Our all-in-one system combines all stages, including single-cell isolation, single-cell productivity screening, and phenotype selection in a single step. This process speeds up biologics production or drug discovery.
• Antibody discovery typically involves finding specific monoclonal antibodies (mAbs) for diagnosing and treating diseases by targeting a particular epitope. The complete process can be easily optimized and accelerated by our colony picking systems.
• Documentation of clonality is a crucial step in cell line development and monoclonality assurance. During single cell experiments different cell types are imaged and selected for easy regulatory filings.
• Using high-resolution imaging, we can pinpoint desirable clonal stem cell colonies for efficient colony screening and selection during discrete colony formation. The feeder-free, adherent cells can be transferred gently to high-density plates with specialized picking pins, ensuring clonal expansion and subsequent analysis.

FAQs