FlexStation® 3 Multi-Mode Microplate Reader
Precise optics, fluidic transfer, and assay flexibility on one integrated microplate reader
Automated pipetting ensures consistent performance over multiple assay conditions on a microplate - FlexStation® 3 Multi-Mode Microplate Reader
The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics.
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Enable more assay conditions
Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate.
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Automate pipetting
Improve assay quality and increase throughput with automated pipetting ensuring consistent addition time and minimizing errors, thus providing tighter assay CVs within and between experiments.
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Validate your reader
Get fluid transfer validation with our PathCheck Feature to quantify pipettor head performance. Use it with SoftMax® Pro Software and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance.
Key Features of FlexStation® 3 Multi-Mode Microplate Reader
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Onboard pipettor
Integrated 8-channel or 16-channel pipettor increases assay flexibility by transferring reagents from 96 or 384 distinct wells in a source plate to the read plate.
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Optimal assay excitation
Dual monochromators allow users to target the optimal assay excitation and emission wavelengths and eliminate the need to change expensive band pass filters between experiments.
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Low volume applications
SpectraDrop™ Micro-Volume Microplate offers a high-throughput solution for low volume measurement, accelerating sample preparation time and increased productivity in DNA, RNA and protein research.
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Robot integration
The FlexStation 3 reader can be integrated with all leading partner robots and with our out-of-the-box automation solution for savings on up-front integration time and resources.
Applications of FlexStation 3 Multi-Mode Microplate Reader
- Absorbance
- Action Potential
- Action Potential Analysis
- Cellular Signaling
- Drug Discovery & Development
- ELISA
- Enzyme Activity
- Fast Kinetic Calcium Mobilization Assays
- Fluorescence
- Fluorescence Polarization (FP)
- IgG Quantification
- Luminescence
- Microbiology and Contaminant
- Protein Detection, Quantitation and Analysis
- Reporter Gene Assay
- TRF, TR-FRET & HTRF
| Item Height Range (Metric cm) | 48 cm |
| Item Width Range (Metric cm) | 58 cm |
| Microplate Reader Type | Multi-Mode Reader |
| Detection/Read Mode(s) | Absorbance, Fluorescence Intensity (Top/Bottom Read), Luminescence (Top/Bottom Read), Time-Resolved Fluorescence (Top/Bottom Read), Fluorescence Polarization (Top Read), Homogenous Time-Resolved Fluorescence (Top/Bottom Read) |
| Detection/Read Type(s) | Endpoint, Kinetic, Spectrum Scan, Well Scan |
| Absorbance Read Time Range (sec) | 17 sec |
| Fluorescence Intensity Read Time Range (sec) | 15 sec |
| Luminescence Read Time Range (sec) | 17 sec |
| Fluorescence Polarization Read Time Range (sec) | 44 sec |
| Time-Resolved Fluorescence Read Time Range (sec) | 15 |
| Minimum Absorbance Wavelength Range (Metric nm) | 200 nm |
| Maximum Absorbance Wavelength Range (Metric nm) | 1000 nm |
| Minimum Fluorescence Intensity Wavelength Range (Metric nm) | 250 nm |
| Maximum Fluorescence Intensity Wavelength Range (Metric nm) | 850 nm |
| Minimum Luminescence Wavelength Range (Metric nm) | 250 nm |
| Maximum Luminescence Wavelength Range (Metric nm) | 850 nm |
| Minimum Time-Resolved Fluorescence Wavelength Range (Metric nm) | 250 nm |
| Maximum Time-Resolved Fluorescence Wavelength Range (Metric nm) | 850 nm |
| Minimum Fluorescence Polarization Wavelength Range (Metric nm) | 400 nm |
| Maximum Fluorescence Polarization Wavelength Range (Metric nm) | 750 nm |
| Fluorescence Intensity Sensitivity Top Read Optimization | 1 fmol/well in 200 μL FITC 96 well (< 5 pM), 2 fmol/well in 100 μL FITC 384 well (< 20 pM) |
| Luminescence Sensitivity Top Read Optimization | < 2 fg/well for firefly luciferase in 96 and 384 well |
| Time-Resolved Fluorescence Sensitivity Top Read Optimization | 100 fM europium in 96 and 384 well |
| Fluorescence Polarization Sensitivity Top Read Optimization | < 5 mP standard deviation at 1 nM fluorescein in 96 and 384 well |
| AlphaScreen Sensitivity Top Read Optimization | - |
| Microplate Photometric Accuracy | < ±1.0% and ±0.003 OD (0.0-2.0 OD) |
| Cuvette Photometric Accuracy | - |
| Photometric Precision | < ±1.0% and ±0.003 OD (0.0-2.0 OD) |
| Microplate Reader Detector(s) | Silicon Photodiode, Photomultiplier Tube |
| Light Source(s) | Xenon Flash Lamp |
| Microplate Size Compatibility | 6 well plate, 12 well plate, 24 well plate, 48 well plate, 96 well plate, 384 well plate |
| Microplate Shaking Mode(s) | Linear |
| Stray Light Measurement | - |
| Wavelength Selection | Monochromator tunable in 1 nm increments |
| Microplate Reader Validation Tool(s) | Absorbance Validation Plate, Fluorescence Validation Plate, Luminescence Validation Plate, SoftMax® Pro GxP Software |
| Includes Validation Tools | Yes |
| Includes Injectors | Yes |
| Includes Temperature Control Capability | Yes |
| Minimum Temperature Control Range Added to Ambient Temperature (Metric °C) | 4 °C |
| Maximum Temperature Control Range (Metric °C) | 45 °C |
| Item Depth Range (Metric cm) | 41 cm |