Activation of CD3⁺ T Cells for Autologous CAR-T Cell Therapy
T cell activation is required for genetic modification and is performed with in vitro exposure to activating ligands. Track cell viability numbers with the Beckman Coulter Life Sciences Vi-CELL BLU Cell Viability Analyzer. The Beckman Coulter Life Sciences Multisizer 4e Coulter Size Analyzer can measure cell volume changes after activation and supports GMP compliance.
Phenotypic assays requiring microplate imaging readouts can be performed using a Molecular Devices SpectraMax Multi-mode Reader with Softmax Pro GxP software. Sample preparation can be performed on a Beckman Coulter Life Sciences Biomek i-Series Automated Workstation that supports 21 CFR Part 11. The Leica Microsystems STELLARIS Confocal Microscope Platforms enable live cell and time-lapse imaging experiments for phenotypic assays. Collect and track experiment and instrument data within discovery or development labs with the IDBS Polar Biopharma Lifecycle Management software.
To enhance GMP manufacturing, the IDBS Skyland PIMS platform can be used for a 21 CFR Part 11 compliant digital backbone and end-to-end analytics workspace. By doing so, it is possible to seamlessly combine product and process data, resulting in reduced production costs, fewer batch failures and easier regulatory reporting. In GMP-compliant environments, monitoring can be performed with a Beckman Coulter Life Sciences MET ONE 3400+ Series Portable Airborne Particle Counter and Beckman Coulter Life Sciences Anatel PAT700 Total Organic Carbon Analyzer.