Our ELISA antibodies are meticulously engineered to deliver reliable results across diverse research applications. Designed to detect antigens from various species, these antibodies provide researchers with a robust and reproducible tool for their studies.
We offer recombinant monoclonal antibodies known for their superior batch-to-batch consistency. Unlike traditional polyclonal antibodies, recombinant monoclonal antibodies exhibit precise targeting, reducing variability and enhancing experimental reliability. Whether studying disease pathways, signaling mechanisms, or biomarker identification, these antibodies serve as indispensable assets for generating accurate and reproducible data.
Our product range includes antibodies tagged with biotin, FITC, PE, and HRP, catering to diverse detection methods. These tags facilitate efficient binding and visualization in multiple assay formats, ensuring compatibility with ELISA. Additionally, our antibodies are derived from various host species, including rabbits, mice, goats, and rats, allowing for compatibility with various experimental systems and reducing species-related cross-reactivity concerns.
Our catalog includes antibodies that can detect proteins with post-translational modifications, including phosphorylation and acetylation. These modifications play crucial roles in cellular signaling and gene regulation, and our antibodies ensure precise detection, supporting studies on protein function and disease mechanisms.
Our carrier-free formulations provide researchers with the flexibility to conjugate antibodies to their preferred labels, adapting seamlessly to experimental needs. Whether researchers need isotype-specific antibodies or require trial sizes for preliminary testing, our extensive catalog offers solutions tailored to diverse workflows.
We also provide matched antibody pairs, which are essential for sandwich ELISAs. These pairs, comprising a capture antibody and a detection antibody, offer highly specific protein recognition with distinct antigens that have no overlap, ensuring optimal sensitivity and accuracy in detecting target molecules. Our matched antibody pairs undergo rigorous screening in plasma and serum, guaranteeing specificity even in complex biological samples.
Whether conducting functional studies, biomarker validation, or protein analysis, our ELISA antibodies set a new benchmark in experimental precision.
Features
- Our recombinant monoclonal antibodies ensure high specificity and batch-to-batch consistency.
- They offer wide species compatibility, making them suitable for detecting antigens from human, mouse, and rat samples in various applications.
- A variety of conjugation options are available, including biotin, FITC, PE, and HRP, catering to different experimental needs.
- They support the recognition of key modifications, including phosphorylation and acetylation of residues, enabling precise detection of post-translational modifications.
- Carrier-free formulations allow researchers to perform custom conjugation, providing flexibility for experimental designs.
- A diverse selection of isotypes and trial sizes ensures suitability for various research applications and preliminary studies.
Applications
- Quantitative ELISA: Our antibodies support quantitative ELISA by functioning as both capture and detection agents, enabling accurate antigen quantification in serum, plasma, and cell culture supernatants.
- Sandwich ELISA: Matched antibody pairs provide high specificity and sensitivity in sandwich ELISAs, ideal for detecting multi-epitope proteins in complex biological samples.
- Competitive ELISA: Suitable for competitive ELISAs, our antibodies enable the detection of small molecules and limited-epitope antigens, such as hormones, drugs, and metabolites.
- Direct ELISA: Our antibodies are compatible with direct ELISAs, where labeled antibodies detect immobilized antigens. Ideal for fast, high-throughput screening.
- Indirect ELISA: In indirect ELISAs, our primary antibodies bind to the target antigens, followed by enzyme-linked secondary antibodies for enhanced signal sensitivity.
- Detection of post-translational modifications: Designed for specificity, our antibodies detect key post-translational modifications, such as phosphorylation and acetylation, which are critical for signaling and gene regulation studies.
- High sensitivity: Using recombinant monoclonal antibodies, our ELISA kits reliably detect low-abundance proteins essential for biomarker research.
- Custom conjugation: Carrier-free formulations allow for custom conjugation, giving researchers the flexibility to design ELISAs tailored to their experimental needs.
- Validation in complex samples: Our antibodies are rigorously validated in serum and plasma, ensuring consistent specificity and sensitivity across complex biological matrices.
What are ELISA antibodies?
ELISA antibodies are antibodies validated for use in ELISA to detect and bind to target antigens with high specificity and batch-to-batch consistency. They are essential for accurate protein quantification, enabling researchers to study disease markers, immune responses, and biological pathways.
How are ELISA antibodies utilized in research?
ELISA antibodies play a crucial role in multiple research techniques, including functional studies, ELISA, Western blot, and flow cytometry. They help scientists investigate cellular functions, signaling pathways, and protein interactions, providing insights into disease mechanisms and potential therapeutic targets. Their ability to recognize specific antigens ensures precise experimental results.
What types of tags are available for ELISA antibodies?
The different types of tags available for ELISA antibodies include biotin, FITC, PE, and HRP, each tailored for different research needs. These tags enhance visualization, detection sensitivity, and experimental flexibility, allowing researchers to choose the optimal format for their assays. Whether for colorimetric detection, fluorescence-based analysis, or enzyme-linked amplification, tagged antibodies streamline workflows.
Do ELISA antibodies come in carrier-free formulations?
Yes, our ELISA antibodies are available in carrier-free formulations, making them ideal for custom conjugation. Without interfering with substances like BSA, glycerol, or azide, researchers can modify antibodies to fit specific experimental conditions, such as linking them to alternative tags or combining them with specialized detection systems. This flexibility allows for tailored assay designs.
What are matched antibody pairs?
Matched antibody pairs consist of a capture antibody and a detection antibody with distinct antigens with no overlap, working together in sandwich ELISAs to bind to the target protein with high specificity and sensitivity. These pairs undergo rigorous screening in plasma and serum, ensuring optimal performance even in complex samples. Their precise interaction facilitates accurate quantification of low-abundance proteins, making them indispensable for biomarker research.