Our recombinant monoclonal antibodies are engineered for exceptional performance by cloning antibody-coding genes into high-yield mammalian expression vectors. These vectors are then introduced into expression hosts, such as HEK 293 or CHO cell lines, to produce functional antibodies with accurate post-translational modifications.

Recombinant monoclonal antibody development can be achieved through hybridoma conversion or phage display technology. In hybridoma conversion, mRNA from antibody-producing hybridomas is isolated, sequenced, and expressed in mammalian cell lines like HEK 293 to ensure greater consistency and specificity. Phage display technology, on the other hand, enables rapid antibody discovery without animal immunization by screening phage libraries against target antigens, enriching specific binders, and engineering the antibody genes into mammalian expression systems for production. These antibodies undergo rigorous validation through techniques like ELISA, western blot, and immunohistochemistry, with tissues from multiple disease types and normal tissues, as well as using knock-out cell lines to ensure optimal performance. Whether monoclonal, multiclonal, or chimeric, our recombinant monoclonal antibodies redefine precision and reliability.

With identical variable regions that target a specific epitope on an antigen, our recombinant monoclonal antibodies deliver sensitivity, specificity, and precision. They ensure consistent results across batches, making them ideal for applications requiring high reproducibility.

For applications that demand greater versatility, our recombinant multiclonal antibodies offer a defined mixture of individually selected recombinant monoclonal antibodies. Each antibody in the mix is designed to recognize different epitopes on the same antigen, combining the broad epitope coverage of polyclonal antibodies with the reproducibility and specificity of recombinant monoclonals, as well as the batch-to-batch consistency.

With our range of recombinant antibodies, you gain unmatched performance, enhanced sensitivity, and the confidence to tackle even the most challenging targets with ease.

FEATURES

• Our recombinant monoclonal antibodies are engineered for exceptional reliability, ensuring batch-to-batch consistency through the use of a defined set of genes. This results in reproducible performance and reliable results for faster project progression, as demonstrated by the consistent affinity (low KD values).
• Our recombinant monoclonal antibodies are validated through rigorous industry-leading methods, including knock-out validation and testing across several applications, sample types, and disease states, ensuring unmatched specificity. By optimizing the selection process at both the hybridoma and recombinant cloning stages, we deliver antibodies with superior qualities tailored to your needs. With remarkably high affinities in the picomolar range (10⁻¹⁰–10⁻¹²), these antibodies provide enhanced sensitivity, binding strongly to antigens and maintaining stability under challenging conditions. This high performance ensures reliable results.
• Recombinant antibody manufacturing enables scalable production by cloning antibody genes into expression vectors, ensuring a consistent and reliable supply. This makes them ideal for long-term studies and projects requiring the same antibody across multiple samples.
• Our high-throughput in vitro manufacturing process ensures efficient production once antibody-producing genes are isolated. For antibodies developed with our phage display technology, this process is entirely animal-free, providing a sustainable and ethical solution.

APPLICATIONS

• Multiplex IHC (mIHC): Our recombinant monoclonal antibodies enable precise multiplex staining, ensuring batch-to-batch consistency in tissue analysis.
• Immunocytochemistry/immunofluorescence (ICC/IF): Our recombinant antibodies provide high specificity and reproducibility for detecting proteins in cultured cells.
• Western blot: These antibodies enhance target detection, minimizing background noise and off-site binding, ensuring consistent results.
• Immunohistochemistry-paraffin (IHC-P): Our recombinant antibodies offer reliable staining in FFPE tissues, improving diagnostic accuracy.
• Flow cytometry: intracellular staining (Flow Cyt (Intra)): Our recombinant antibodies are designed to allow sensitive detection of intracellular markers with minimal variability.
• Immunoprecipitation (IP): Our antibodies allow for efficient protein pull-down with specificity in complex lysates.
• Chromatin immunoprecipitation (ChIP): These antibodies enhance protein-DNA interaction studies by providing high affinity.
• By blocking protein-protein interactions, recombinant monoclonal antibodies can be used to unravel the mechanism of action of a biochemical process. Single-chain variable fragments are particularly useful in probing intracellular pathways.
• Recombinant antibody technology provides a versatile and scalable platform, transforming research by ensuring high specificity, reproducibility, and adaptability for complex biological challenges.

FAQs