Our comprehensive selection of conjugated antibodies, particularly fluorescent conjugated primary antibodies, are designed to enhance the accuracy and efficiency of your experimental needs. With our expanding collection, researchers can confidently build multiplex panels using high-performance conjugates that deliver reliable and consistent results.
We rigorously validate our conjugated antibodies across key applications, such as flow cytometry, immunocytochemistry, and immunohistochemistry in multi-normal and multi-disease tissue types and samples. By ensuring thorough validation, we provide researchers with confidence in their experimental outcomes, reducing variability and improving data reliability. Our primary conjugates for flow cytometry undergo additional testing in ICC/IF to confirm signal localization, further enhancing their accuracy.
Batch-to-batch consistency is crucial for reproducible results, and our stringent quality control measures and recombinant manufacturing technologies ensure minimal experimental variability. By maintaining a high signal-to-noise ratio, we optimize the specificity and sensitivity of assays, reducing issues like cross-talk. This commitment to quality allows researchers to focus on their studies rather than troubleshooting inconsistencies.
Our pre-validated fluorescent-labeled antibodies eliminate the need for extensive in-house validation, streamlining workflows and allowing for more efficient research. With high specificity and accuracy, these primary conjugated antibodies bind directly to their targets, reducing background noise and minimizing the risk of false-positive results.
If you require customized solutions, we provide tailored antibody conjugation services to meet specific experimental needs. Our expert team will work closely with you to deliver the ideal conjugate for your assays. Available labels for custom conjugation include Alexa Fluor dyes, Phycoerythrin, Allophycocyanin, FITC, Biotin, HRP, Alkaline Phosphatase, Gold nanoparticles, and Oligonucleotide/DNA barcodes.
Choosing the right fluorophore is essential for obtaining accurate and reproducible results. Our extensive selection of antibody conjugation solutions includes CF405M, CY5, CY7, Dylight 488, Dylight 550, Dylight 650, RedFluor 710, Rhodamine, SureLight APC, Texas Red, VioletFluor 450, and VioletFluor 500. This wide range ensures that researchers can find the most suitable option for their specific applications.
With our high-quality fluorescent conjugated antibodies, researchers can achieve bright, specific, and reproducible results, advancing their immunofluorescence and flow cytometry studies with confidence.
With over 35 conjugates to choose from, a wide selection of carrier-free antibodies suitable for conjugation, our Lightning Link® kits for quick and simple conjugation, and a custom conjugation service, using Abcam, you have the ultimate flexibility in antibody choice for your experimental needs.
Explore our full range of conjugated antibodies and conjugation kits and discover the ideal solutions for your research needs.
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Features
- Reliable & high-sensitivity solutions: Our fluorescent conjugated antibodies ensure specificity and accuracy, eliminating false positives and providing consistent, high-quality results for immunofluorescence and flow cytometry.
- Save time & streamline your workflow: With a single incubation step, our wide range of conjugated antibodies reduce in-house validation time, helping you focus on advancing your research.
- Wide fluorophore selection for multiplexing : Choose from an extensive range, including Alexa Fluor dyes, FITC, PE, and APC, to achieve high-resolution detection tailored to your experimental needs.
Applications
- Western blot: Typically uses horseradish peroxidase (HRP), alkaline phosphatase (AP), or fluorescent dye-conjugated antibodies.
- Immunofluorescence: Employs antibodies conjugated to fluorescent dyes (eg, Alexa Fluor).
- Fluorescent immunohistochemistry, singleplex: Uses antibodies labeled with a fluorescent dye.
- Fluorescent immunohistochemistry, multiplex: Relies on multiple fluorescent dyes or oligonucleotide-conjugated antibodies.
- Chromogenic immunohistochemistry: Utilizes HRP, AP, or biotin-labeled antibodies.
- Flow cytometry: Uses antibodies conjugated to fluorescent dyes (eg, Alexa Fluor), fluorescent proteins (phycoerythrin, allophycocyanin), or tandem dyes to analyze and sort cell populations based on marker expression.
- ELISA & ELISA-based applications: Involves HRP- or biotin-conjugated antibodies.
- Lateral flow assay: Uses antibodies conjugated with latex or gold nanoparticles and sometimes fluorescent nanoparticles (e.g., Europium).
What is an antibody conjugate?
An antibody conjugate is an antibody chemically linked to a specific label, such as a fluorescent dye or enzyme, that enables the detection, visualization, or quantification of a target antigen in various assays.
What is the difference between a conjugated and unconjugated antibody?
A conjugated antibody has a detectable label (a fluorescent dye or enzyme) chemically attached to it, whereas an unconjugated antibody lacks any direct label and typically requires a secondary detection method.
How are conjugated antibodies used in flow cytometry?
In flow cytometry, conjugated antibodies (typically labeled with fluorescent dyes or proteins) bind specific cell surface or intracellular antigens, and when these labeled cells pass through the cytometer’s laser, the fluorophores are excited and emit light that is detected, thereby allowing for the identification and quantification of various cell populations and their protein expression profiles.
What is the role of conjugated antibodies in immunofluorescence microscopy?
In immunofluorescence microscopy, conjugated antibodies specifically bind to target antigens within cells or tissues and, due to their attached fluorescent label, allow visualization of the antigen’s location and distribution when excited by the appropriate wavelength of light.
What are the benefits of using conjugated antibodies?
Conjugated antibodies simplify detection protocols, enhance specificity, and enable multiplexing by directly linking a detectable label, thereby saving time and improving sensitivity in various immunoassays.