Plasmid Vector Construction
Vector construction requires piecing together different elements, such as DNA sequences for the gene of interest, promoters, a selectable marker (if required) and regulatory elements into an expression vector. Once assembled, the resultant recombinant plasmid is subjected to quality control checks and cloning to generate significant copies for downstream steps.
Plasmid construction and molecular cloning routinely leverage Golden Gate and Gibson assembly to ligate oligos. Whether you prefer to outsource or keep the plasmid construction workflow in-house, Integrated DNA Technologies can support your needs with a full range of gene products and services. The Beckman Coulter Life Sciences Biomek Echo One can facilitate the automation of rapid DNA/plasmid assembly. The Molecular Devices QPix 400-Series Microbial Colony Picker can automate the picking and streaking of bacterial cell cultures, producing the desired plasmid.
Digital workflows for structured data capture can increase efficiency and save significant time. The IDBS E-WorkBook and Polar BioPharma Lifecycle Management platform can support data capture and integration for streamlined reporting and data sharing with internal or external teams.