Sequence Verification for Cell Line Development
After constructing plasmids, they must be analyzed to ensure they produce the desired antibodies or proteins. It’s important to score plasmids based on criteria like sequence identity. PCR and NGS for DNA sequencing are common tools leveraged for sequence verification.
PCR can determine plasmid sequence identity by leveraging complementary probes for the inserted gene and vector elements. PCR workflows can be automated on a Beckman Coulter Life Sciences Biomek i-Series Automated Workstation.
Automated DNA sequencing workflows benefit from the bead-based automation-friendly Beckman Coulter Life Sciences Plasmid Purification Kit. For high-throughput genomic sample preparation, including NGS sequencing library preparation steps like extraction, fragmentation, ligation, normalization and pooling, leverage the Beckman Coulter Life Sciences Biomek Echo One. Navigate the complex and abundant reads to choose mutation-free winners and generate sequencing reports with Genedata Selector Software.
Digital workflows for structured data capture can increase efficiency and save significant time. The IDBS E-WorkBook and Polar BioPharma Lifecycle Management platform can support data capture and integration for streamlined reporting and data sharing with internal or external teams.