Anti-PD1 Antibody (ab52587) | Danaher Life Sciences

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

Immunohistochemical analysis of human large and locally advanced breast cancers staining PD1 using ab52587.

(Panel a) Low level of PD-1+ T cell infiltration.

(Panel b) high level of PD-1+ T cell infiltration. (Itu: intratumoral Str: stromal).

Image data from PMID: 27777963

This image is from PubMedId: 27777963. Kaewkangsadan V et al. (2016) Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Immunohistochemical staining of human breast cancer tissue for PD-1 expression, showing varying levels of PD-1+ T cell infiltration in tumor and stromal regions.

Flow Cytometry - Anti-PD1 antibody [NAT105] (ab52587)

Sample: Human tonsil cell extract.

Dilution: ab52587 antibody was used as 1/200 in 1x106 cells/tube.

Anti-CD4 antibody was used as 1/200 dilution.

Anti-CD8 antibody was used as 1/200 dilution.

Flow cytometry analysis of human tonsil cell extract stained with anti-PD1 antibody (ab52587)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

Expression of markers of T cell differentiation and degree of inflammation in the heart of chronically T. cruzi-infected subjects with severe cardiomyopathy.

A–D, left panel: representative photos of CD45RO, CD27, PD1 and CD57 expression, respectively.

Panel C shown.

Image data from PMID: 25144227

Argüello et al PLoS Negl Trop Dis. 2014 Aug 21;8(8):e2989. doi: 10.1371/journal.pntd.0002989. eCollection 2014 Aug. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunohistochemistry image showing PD1 expression in heart tissue of a patient with chronic Chagas disease.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

Double immunofluorescence staining of CD3 (green) and PD1 (red) on paraffin embedded tonsil.

Immunofluorescence staining of CD3 and PD1 proteins in a tonsil tissue section.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105] (ab52587)

Representative immunohistochemical staining results for PD1 using ab52587 at a 1/50 dilution in human formalin-fixed, paraffin-embedded tissue specimens.

Panel A: Normal lung tissue, negative control;

Panel B: Tonsillar tissue, positive control;

Panel C: PD1-negative tumor infiltrating lymphocytes;

Panel D: PD1-positive tumor infiltrating lymphocytes in squamous cell carcinomas).

Image from PMID: 26313362

Schmidt et al PLoS One. 2015 Aug 27;10(8):e0136023. doi: 10.1371/journal.pone.0136023. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/