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Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)

SDS-PAGE image showing molecular weight marker (MW) and His-tag recombinant protein fractions purified in Ni-NTA resin in lanes 1-5 stained with InstantBlue. InstantBlue was easy to handle and use - just pour a small amount onto your gel and within 10 minutes you can see the protein bands! No methanol and glacial acetic acid - no smell!

This image is courtesy of an anonymous customer review.

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)

Staining - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)

Staining - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)

SDS-PAGE - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)

Lane 1: Recombinant Human GAPDH protein (ab77109) at 3 ug + Instant Blue stain (ab119211)

Lane 2: Recombinant Human GAPDH protein (ab77109) at 2 ug + Instant Blue stain (ab119211)

Lane 3: Recombinant Human GAPDH protein (ab77109) at 1 ug + Instant Blue stain (ab119211)

Lane 4: Recombinant Human GAPDH protein (ab77109) at 0.5 ug + Instant Blue stain (ab119211)

Lane 5: Recombinant Human GAPDH protein (ab77109) at 0.3 ug + Instant Blue stain (ab119211)

Lane 6: Recombinant Human GAPDH protein (ab77109) at 0.2 ug + Instant Blue stain (ab119211)

Lane 7: Recombinant Human GAPDH protein (ab77109) at 0.1 ug + Instant Blue stain (ab119211)

Predicted band size: 36 kDa

Observed band size: 36 kDa

SDS-PAGE image showing molecular weight marker (MW) and Recombinant Human GAPDH protein in lanes 1-7 stained with InstantBlue. The gel was stained for 30 minutes and then rinsed once with distilled water to remove the stain.

SDS-PAGE - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
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