InstantBlue® Coomassie Protein Stain (ISB1L)(AB119211)
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Coomassie InstantBlue® Protein Stain is a ready to use protein stain for polyacrylamide gels.
Rapid staining with well-defined blue bands in 15 minutes or less
Single step procedure eliminating the need to wash, fix or destain
High sensitivity detecting bands down to 5ng
Clear background with high signal: noise ratio
Reliable – consistent batch to batch staining
Efficient – Only 25 ml per gel
Non-toxic composition so no fume hood or solvent disposal is required.
Methanol and acetic acid free - No gel shrinkage or protein methylation/acetylation
Compatible with Mass spectrometry protein identification
Coomassie InstantBlue® Protein Stain (ISB1L) is cited in over 490 publications.
Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
SDS-PAGE image showing molecular weight marker (MW) and His-tag recombinant protein fractions purified in Ni-NTA resin in lanes 1-5 stained with InstantBlue. InstantBlue was easy to handle and use - just pour a small amount onto your gel and within 10 minutes you can see the protein bands! No methanol and glacial acetic acid - no smell!
This image is courtesy of an anonymous customer review.
Staining - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
SDS-PAGE - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
Lane 1: Recombinant Human GAPDH protein (ab77109) at 3 ug + Instant Blue stain (ab119211)
Lane 2: Recombinant Human GAPDH protein (ab77109) at 2 ug + Instant Blue stain (ab119211)
Lane 3: Recombinant Human GAPDH protein (ab77109) at 1 ug + Instant Blue stain (ab119211)
Lane 4: Recombinant Human GAPDH protein (ab77109) at 0.5 ug + Instant Blue stain (ab119211)
Lane 5: Recombinant Human GAPDH protein (ab77109) at 0.3 ug + Instant Blue stain (ab119211)
Lane 6: Recombinant Human GAPDH protein (ab77109) at 0.2 ug + Instant Blue stain (ab119211)
Lane 7: Recombinant Human GAPDH protein (ab77109) at 0.1 ug + Instant Blue stain (ab119211)
Predicted band size: 36 kDa
Observed band size: 36 kDa
SDS-PAGE image showing molecular weight marker (MW) and Recombinant Human GAPDH protein in lanes 1-7 stained with InstantBlue. The gel was stained for 30 minutes and then rinsed once with distilled water to remove the stain.
| Shipped At Conditions | Ambient - Can Ship with Ice |
| Appropriate Long term Storage Conditions | Store at +4°C. |