Tagged proteins are essential tools in modern research. With various tagging options, our recombinant proteins provide the versatility needed for different experimental workflows.

Purification tags: Tags like His-tag, GST, and FLAG make the purification process much simpler and more efficient. These tags allow proteins to be isolated quickly and specifically by using affinity chromatography. They bind only to certain ligands and make it easier to separate the protein of interest or the target protein from the rest of the cellular material. This saves time and improves the overall purity of the final protein sample.

Solubility and stability tags: Tags such as maltose-binding protein (MBP) and glutathione S-transferase (GST) are added to proteins to improve their solubility and stability. This means the proteins are less likely to form aggregates or degrade during experiments, making them much easier to work with. Maintaining protein integrity is crucial for achieving consistent and reliable results, especially in challenging experimental conditions.

Detection and quantification tags: Certain tags make it easier to detect and measure proteins in different types of assays, such as western blotting, immunoprecipitation, and flow cytometry. Tagged proteins are particularly useful for studying how proteins interact with each other, which is essential for uncovering the intricacies of cellular processes and signaling pathways. This ability to track and quantify proteins accurately plays a key role in both basic research and clinical studies.

Structural analysis tags: Tags are also valuable tools for structural studies, helping researchers crystallize proteins and analyze their three-dimensional structures. Understanding a protein’s 3D shape is vital for learning how it functions and for designing targeted therapeutics. Structural tags streamline this process, making it easier to obtain high-quality data for pre-clinical drug development and biological research.

FEATURES

• Wide range of tags: Our catalog offers a wide selection of protein tags, including options such as His, GST, Fc, Avi, FLAG, His-DHFR, His-T7, T7, His-DDDDK, 10x His, Myc, 6x His, and SUMO. This variety ensures that researchers can choose the best tag for their specific experimental goals, whether they need easier purification, improved stability, or specialized detection methods.
• Recombinant proteins: All our tagged proteins are recombinantly or synthetically developed, meaning they are produced using controlled expression systems to ensure high purity and reliable consistency from batch to batch. This helps researchers achieve more reproducible results and reduces variability in their experiments.
• Enhanced solubility and stability: Certain tags, like MBP and GST, are specifically chosen to enhance the solubility and stability of proteins. This makes the proteins easier to work with, prevents aggregation, and helps maintain their functional activity during experiments.
• Efficient purification: His-tagged and FLAG-tagged proteins allow for quick and straightforward purification through affinity chromatography. These tags bind specifically to purification resins, allowing researchers to isolate their proteins of interest with high purity while minimizing sample loss. This is particularly useful when studying protein-protein interactions.
• Versatile detection: Tagged proteins are incredibly useful for detection and quantification in a range of assays, including western blotting, immunoprecipitation, and flow cytometry. The presence of a tag allows researchers to easily track, measure, and analyze proteins across different experimental platforms.

APPLICATIONS

• Affinity purification: Tags such as His, GST, GFP, and FLAG are widely used for protein purification through affinity chromatography. These tags allow researchers to selectively isolate their proteins of interest from complex mixtures with high purity and minimal loss.
• Protein detection: Protein tags facilitate easy detection and quantification in various assays, including Western blotting, immunoprecipitation, and flow cytometry. This enables accurate monitoring of protein expression, localization, and interactions across different experimental systems.
• Protein interaction studies: Tagged proteins are valuable tools for studying interactions with other proteins, providing critical insights into cellular processes and signaling pathways. Exploring these interactions is essential for understanding the mechanisms that drive biological functions and disease.
• Structural biology: Protein tags also play a key role in structural biology by aiding in the crystallization and structural analysis of proteins. Determining the three-dimensional structure of proteins helps reveal their function and supports the development of targeted therapeutic strategies.

FAQs