ATTO 680
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ATTO 680 belongs together with ATTO 655 and ATTO 700 to a new generation of fluorescent labels.
Optical properties
- λ abs = 681 nm
- ε max = 1.25×105 M -1 cm -1
- λ fl = 698 nm
- n fl = 30 %
- τ fl = 1.7 ns
- CF 260 = ε 260 /ε max = 0.30
- CF 280 = ε 280 / ε max = 0.17
ATTO 680 belongs together with ATTO 655 and ATTO 700 to a new generation of fluorescent labels.
Optical properties
- λ abs = 681 nm
- ε max = 1.25×105 M -1 cm -1
- λ fl = 698 nm
- n fl = 30 %
- τ fl = 1.7 ns
- CF 260 = ε 260 /ε max = 0.30
- CF 280 = ε 280 / ε max = 0.17
ATTO 680 belongs together with ATTO 655 and ATTO 700 to a new generation of fluorescent labels.
Characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and high thermal and photo-stability. ATTO 680 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 680 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.
The fluorescence is excited most efficiently in the range 645 - 695 nm. A suitable excitation source for the dye is the Krypton-Ion laser using the 676 nm line or a diode-laser emitting at 670 nm.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems