ATTO 680 Tetrazine (MeTet) Fluorescent Label 0,2mg

Optical properties

• λ _abs = 681 nm
• ε _max = 1.25×10⁵ M ^-1 cm ^-1
• λ _fl = 698 nm
• n _fl = 30 %
• τ _fl = 1.7 ns
• CF ₂₆₀ = ε ₂₆₀ /ε _max = 0.30
• CF ₂₈₀ = ε ₂₈₀ / ε _max = 0.17

ATTO 680 belongs together with ATTO 655 and ATTO 700 to a new generation of fluorescent labels.

Characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and high thermal and photo-stability. ATTO 680 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 680 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.

The fluorescence is excited most efficiently in the range 645 - 695 nm. A suitable excitation source for the dye is the Krypton-Ion laser using the 676 nm line or a diode-laser emitting at 670 nm.