ATTO 665
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ATTO 665 is a new fluorescent label related to ATTO 647N.
Optical properties
- λ abs = 662nm
- ε max = 1.60×105 M -1 cm -1
- λ fl = 680 nm
- n fl = 60 %
- τ fl = 2.9 ns
- CF 260 = ε 260 /ε max = 0.07
- CF 280 = ε 280 / ε max = 0.06
ATTO 665 is a new fluorescent label related to ATTO 647N.
Optical properties
- λ abs = 662nm
- ε max = 1.60×105 M -1 cm -1
- λ fl = 680 nm
- n fl = 60 %
- τ fl = 2.9 ns
- CF 260 = ε 260 /ε max = 0.07
- CF 280 = ε 280 / ε max = 0.06
ATTO 665 is a new fluorescent label related to ATTO 647N.
Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. In common with most ATTO-TEC-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications.
ATTO 665 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 665 is a hydrophobic dye. The fluorescence is excited most efficiently in the range 640 - 675 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems