ATTO 665 Phalloidin Fluorescent Label 10 nmol
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Product No. AD-665-81
ATTO 665 is a new fluorescent label related to ATTO 647N.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
Optical properties
- λ abs = 662nm
- ε max = 1.60×105 M -1 cm -1
- λ fl = 680 nm
- n fl = 60 %
- τ fl = 2.9 ns
- CF 260 = ε 260 /ε max = 0.07
- CF 280 = ε 280 / ε max = 0.06
ATTO 665 is a new fluorescent label related to ATTO 647N.
Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications.
ATTO 665 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 665 is a hydrophobic dye. The fluorescence is excited most efficiently in the range 640 - 675 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
| Modification | Phalloidin |
| Unit of Measure | 10 nmol |
| Product Group | ATTO 665 |
| Product Type | Fluorescent Label |
| Emission Line (nm) | 647, 676 |
| Fluorescence Maximum (nm) | 680 |
| MH+ (g/mol) | 1392 |
| Molecular Weight (g/mol) | 1507 |
| Light Source | Krypton-Ion laser |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-DNA Conjugates (CF260 = e260 /emax ) | 0.07 |
| Correction Factor Used in Calculation of Degree of Labeling (DOL) in Case of Dye-Protein Conjugates (CF280 = e280 /emax) | 0.06 |
| Increase of Molecular Mass on Conjugation with ATTO-label (NHS-ester : amine) | 605.7 |
| Increase of Molecular Mass on Conjugation with ATTO-label (maleimide : thiol) | 745.9 |
| Change of Electrical Charge on Conjugation with ATTO-Label | +1 |
| Longest Wavelength Absorption Maximum (nm) | 662 |
| Molar Decadic Extinction Coefficient at the Longest Wavelength Absorption Maximum (M-1 cm-1) | 160000 |
| Fluorescence Quantum Yield (%) | 60 |
| Real Fluorescence Decay Time (ns) | 2.9 |